Human CLC ELISA Kit

Alternative name
Human Gal-10 ELISA Kit;Human Charcot-Leyden crystal protein ELISA Kit;Human CLC ELISA Kit;Human eosinophil lysophospholipase ELISA Kit;Human lysolecithin acylhydrolase ELISA Kit;Human LGALS10 ELISA Kit;Human LGALS10A ELISA Kit;Human GAL10 ELISA Kit;Human LPPL_HUMAN ELISA Kit;Human Charcot-Leyden crystal galectin ELISA Kit;Human galectin-10 ELISA Kit;Human lectin, galactoside-binding, soluble, 10 ELISA Kit;
Catalog
E007921
species
Human
GeneCLC
SpecificityThis assay has high sensitivity and excellent specificity for detection of Charcot Leyden Crystal Protein (CLC). No significant cross-reactivity or interference between Charcot Leyden Crystal Protein (CLC) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.126ng/mL.
Intended UseHuman CLC ELISA Kit allows for the in vitro quantitative determination of CLC , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Charcot Leyden Crystal Protein (CLC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Charcot Leyden Crystal Protein (CLC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Charcot Leyden Crystal Protein (CLC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Charcot Leyden Crystal Protein (CLC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Galectin-10 Protein information
Uniprot ID LEG10_HUMAN
Uniprot AC Q05315; C5HZ13; C5HZ14; Q0VDE3;
UniGene Hs.889;
GeneID 1178
KEGG hsa:1178;
Human Galectin-10 Protein SEQUENCE
SEQUENCE 142 AA; 16453 MW; 2744F0C082C6446E CRC64;
MSLLPVPYTE AASLSTGSTV TIKGRPLACF LNEPYLQVDF HTEMKEESDI
VFHFQVCFGR RVVMNSREYG AWKQQVESKN MPFQDGQEFE LSISVLPDKY
QVMVNGQSSY TFDHRIKPEA VKMVQVWRDI SLTKFNVSYL KR
UCSC uc002omh.4; human.;

Human CLC ELISA Kit

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