Mouse Prkca ELISA Kit

Alternative name
Mouse PKC-A ELISA Kit;Mouse PKC-alpha ELISA Kit;Mouse Pkca ELISA Kit;Mouse AI875142 ELISA Kit;Mouse protein kinase C, alpha ELISA Kit;Mouse protein kinase C alpha type ELISA Kit;Mouse protein kinase c-alpha ELISA Kit;
Catalog
E065145
species
Mouse
GenePrkca
SpecificityThis assay recognizes recombinant and natural mouse PKC-A. No significant cross-reactivity or interference was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseMouse Prkca ELISA Kit allows for the in vitro quantitative determination of Prkca , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PKC-A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PKC-A and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PKC-A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of PKC-A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse Protein kinase C alpha type Protein information
Uniprot ID KPCA_MOUSE
Uniprot AC P20444;
UniGene Mm.222178;
GeneID 18750
KEGG mmu:18750;
Mouse Protein kinase C alpha type Protein SEQUENCE
SEQUENCE 672 AA; 76852 MW; 394B48C952BB6D50 CRC64;
MADVYPANDS TASQDVANRF ARKGALRQKN VHEVKDHKFI ARFFKQPTFC
SHCTDFIWGF GKQGFQCQVC CFVVHKRCHE FVTFSCPGAD KGPDTDDPRS
KHKFKIHTYG SPTFCDHCGS LLYGLIHQGM KCDTCDMNVH KQCVINDPSL
CGMDHTEKRG RIYLKAEVTD EKLHVTVRDA KNLIPMDPNG LSDPYVKLKL
IPDPKNESKQ KTKTIRSNLN PQWNESFTFK LKPSDKDRRL SVEIWDWDRT
TRNDFMGSLS FGVSELMKMP ASGWYKAHNQ EEGEYYNVPI PEGDEEGNME
LRQKFEKAKL GPVGNKVISP SEDRKQPSNN LDRVKLTDFN FLMVLGKGSF
GKVMLADRKG TEELYAIKIL KKDVVIQDDD VECTMVEKRV LALLDKPPFL
TQLHSCFQTV DRLYFVMEYV NGGDLMYHIQ QVGKFKEPQA VFYAAEISIG
LFFLHKRGII YRDLKLNNVM LNSEGHIKIA DFGMCKEHMM DGVTTRTFCG
TPDYIAPEII AYQPYGKSVD WWAYGVLLYE MLAGQPPFDG EDEDELFQSI
MEHNVSYPKS LSKEAVSICK GLMTKQPAKR LGCGPEGERD VREHAFFRRI
DWEKLENREI QPPFKPKVCG KGAENFDKFF TRGQPVLTPP DQLVIANIDQ
SDFEGFSYVN PQFVHPILQS AV

Category

Mouse Prkca ELISA Kit

Mouse PKC-A ELISA Kit;Mouse PKC-alpha ELISA Kit;Mouse Pkca ELISA Kit;Mouse AI875142 ELISA Kit;Mouse protein kinase C, alpha ELISA Kit;Mouse protein kinase C alpha type ELISA Kit;Mouse protein kinase c-alpha ELISA Kit;