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Alternative name
Platelet-derived growth factor D ELISA KIT; Spinal cord-derived growth factor B ELISA KIT; SCDGF-BPdgfd ELISA KIT; Scdgfb ELISA KIT; PDGF-D ELISA KIT; SCDGF-B ELISA KIT; PDGFD latent form ELISA KIT; PDGFD receptor-binding form ELISA KIT
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Catalog
E063971
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species
Mouse
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GenePDGF-DD
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SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse PDGF-DD. No significant cross-reactivity or interference between mouse PDGF-DD and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.039 ng/ml.
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Intended UseMouse PDGF-DD ELISA Kit allows for the in vitro quantitative determination of PDGF-DD , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PDGF-DD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PDGF-DD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDGF-DD is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDGF-DD bound in the initial step. The color development is stopped and the intensity of the color is measured.
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