Human GBP4 ELISA Kit

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  • Alternative name

    Human GTP-binding protein 4 ELISA Kit;Human GBP-4 ELISA Kit;Human guanine nucleotide-binding protein 4 ELISA Kit;Human Mpa2 ELISA Kit;Human guanylate binding protein 4 ELISA Kit;Human guanylate-binding protein 4 ELISA Kit;

  • Catalog
    E017790
  • species
    Human
  • GeneGBP4
  • Standard CurveHuman GBP4 ELISA Kit
  • Other Species Mouse Gbp4 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 nmol/L.
  • Intended UseHuman GBP4 ELISA Kit allows for the in vitro quantitative determination of GBP4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: Gbp4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Gbp4 antibody and an Gbp4-HRP conjugate. The assay sample and buffer are incubated together with Gbp4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Gbp4 concentration since Gbp4 from samples and Gbp4-HRP conjugate compete for the anti-Gbp4 antibody binding site. Since the number of sites is limited, as more sites are occupied by Gbp4 from the sample, fewer sites are left to bind Gbp4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Gbp4 concentration in each sample is interpolated from this standard curve.
  • Human Guanylate-binding protein 4 Protein information
  • Uniprot ID GBP4_HUMAN
  • Uniprot AC Q96PP9; B2R630; Q05D63; Q6NSL0; Q86T99;
  • UniGene Hs.409925;
  • GeneID 115361
  • KEGG hsa:115361;
  • Human Guanylate-binding protein 4 Protein SEQUENCE
  • SEQUENCE 640 AA; 73165 MW; 06ABFF47E615DAFC CRC64;

    MGERTLHAAV PTPGYPESES IMMAPICLVE NQEEQLTVNS KALEILDKIS

    QPVVVVAIVG LYRTGKSYLM NRLAGKRNGF PLGSTVQSET KGIWMWCVPH

    LSKPNHTLVL LDTEGLGDVE KSNPKNDSWI FALAVLLSSS FVYNSVSTIN

    HQALEQLHYV TELAELIRAK SCPRPDEAED SSEFASFFPD FIWTVRDFTL

    ELKLDGNPIT EDEYLENALK LIPGKNPKIQ NSNMPRECIR HFFRKRKCFV

    FDRPTNDKQY LNHMDEVPEE NLERHFLMQS DNFCSYIFTH AKTKTLREGI

    IVTGKRLGTL VVTYVDAINS GAVPCLENAV TALAQLENPA AVQRAADHYS

    QQMAQQLRLP TDTLQELLDV HAACEREAIA VFMEHSFKDE NHEFQKKLVD

    TIEKKKGDFV LQNEEASAKY CQAELKRLSE HLTESILRGI FSVPGGHNLY

    LEEKKQVEWD YKLVPRKGVK ANEVLQNFLQ SQVVVEESIL QSDKALTAGE

    KAIAAERAMK EAAEKEQELL REKQKEQQQM MEAQERSFQE YMAQMEKKLE

    EERENLLREH ERLLKHKLKV QEEMLKEEFQ KKSEQLNKEI NQLKEKIEST

    KNEQLRLLKI LDMASNIMIV TLPGASKLLG VGTKYLGSRI

  • UCSC uc001dnb.4; human.;


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