Mouse Plg ELISA Kit

Alternative name
Mouse AI649309 ELISA Kit;Mouse Pg ELISA Kit;Mouse plasminogen ELISA Kit;Mouse angiostatin ELISA Kit;Mouse plasmin heavy chain A ELISA Kit;Mouse plasmin light chain B ELISA Kit;
Catalog
E063898
species
Mouse
GenePlg
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseMouse Plg ELISA Kit allows for the in vitro quantitative determination of Plg , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCardiovascular
Product Description
specificalPrinciple of the Assay: PLG ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PLG. Standards or samples are then added to the microtiter plate wells and PLG if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PLG present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for PLG are added to each well to "sandwich" the PLG immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PLG and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PLG concentration in each sample is interpolated from this standard curve.
Mouse Plasmin light chain B Protein information
Uniprot ID PLMN_MOUSE
Uniprot AC P20918; Q8CIS2; Q91WJ5;
UniGene Mm.971;
GeneID 18815
KEGG mmu:18815;
Mouse Plasmin light chain B Protein SEQUENCE
SEQUENCE 812 AA; 90808 MW; E70E1AC8E52844E9 CRC64;
MDHKEVILLF LLLLKPGQGD SLDGYISTQG ASLFSLTKKQ LAAGGVSDCL
AKCEGETDFV CRSFQYHSKE QQCVIMAENS KTSSIIRMRD VILFEKRVYL
SECKTGIGNG YRGTMSRTKS GVACQKWGAT FPHVPNYSPS THPNEGLEEN
YCRNPDNDEQ GPWCYTTDPD KRYDYCNIPE CEEECMYCSG EKYEGKISKT
MSGLDCQAWD SQSPHAHGYI PAKFPSKNLK MNYCRNPDGE PRPWCFTTDP
TKRWEYCDIP RCTTPPPPPS PTYQCLKGRG ENYRGTVSVT VSGKTCQRWS
EQTPHRHNRT PENFPCKNLE ENYCRNPDGE TAPWCYTTDS QLRWEYCEIP
SCESSASPDQ SDSSVPPEEQ TPVVQECYQS DGQSYRGTSS TTITGKKCQS
WAAMFPHRHS KTPENFPDAG LEMNYCRNPD GDKGPWCYTT DPSVRWEYCN
LKRCSETGGS VVELPTVSQE PSGPSDSETD CMYGNGKDYR GKTAVTAAGT
PCQGWAAQEP HRHSIFTPQT NPRAGLEKNY CRNPDGDVNG PWCYTTNPRK
LYDYCDIPLC ASASSFECGK PQVEPKKCPG RVVGGCVANP HSWPWQISLR
TRFTGQHFCG GTLIAPEWVL TAAHCLEKSS RPEFYKVILG AHEEYIRGLD
VQEISVAKLI LEPNNRDIAL LKLSRPATIT DKVIPACLPS PNYMVADRTI
CYITGWGETQ GTFGAGRLKE AQLPVIENKV CNRVEYLNNR VKSTELCAGQ
LAGGVDSCQG DSGGPLVCFE KDKYILQGVT SWGLGCARPN KPGVYVRVSR
FVDWIEREMR NN
UCSC uc008akt.2; mouse.;

Category

Mouse Plg ELISA Kit

Mouse AI649309 ELISA Kit;Mouse Pg ELISA Kit;Mouse plasminogen ELISA Kit;Mouse angiostatin ELISA Kit;Mouse plasmin heavy chain A ELISA Kit;Mouse plasmin light chain B ELISA Kit;