Mouse Rnase2 ELISA Kit

Alternative name
Mouse eosinophil cationic-type ribonuclease 4 ELISA Kit;Mouse MR-4 ELISA Kit;Mouse Ribonuclease 2 ELISA Kit;Mouse RNase 2 ELISA Kit;Mouse Ear4 ELISA Kit;Mouse RNS2 ELISA Kit;Mouse Rnase2 ELISA Kit;Mouse mR4 ELISA Kit;Mouse eosinophil-associated, ribonuclease A family, member 4 ELISA Kit;Mouse non-secretory ribonuclease ELISA Kit;Mouse liver, eosinophil-derived neurotoxin ELISA Kit;Mouse murine ribonuclease 4 ELISA Kit;Mouse ribonuclease, RNase A family, 2 ELISA Kit;
Catalog
E061794
species
Mouse
GeneRnase2
SpecificityThis assay has high sensitivity and excellent specificity for detection of RNASE2. No significant cross-reactivity or interference between RNASE2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RNASE2 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseMouse Rnase2 ELISA Kit allows for the in vitro quantitative determination of Rnase2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyEpigenetics and Nuclear Signaling
Product Description
specificalIntended Uses: This RNASE2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse RNASE2. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||RNASE2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-RNASE2 antibody and an RNASE2-HRP conjugate. The assay sample and buffer are incubated together with RNASE2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RNASE2 concentration since RNASE2 from samples and RNASE2-HRP conjugate compete for the anti-RNASE2 antibody binding site. Since the number of sites is limited, as more sites are occupied by RNASE2 from the sample, fewer sites are left to bind RNASE2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RNASE2 concentration in each sample is interpolated from this standard curve.
Mouse Non-secretory ribonuclease Protein information
Uniprot ID RNAS2_MOUSE
Uniprot AC O35291;
UniGene Mm.377124;
GeneID 53877
KEGG mmu:53877;
Mouse Non-secretory ribonuclease Protein SEQUENCE
SEQUENCE 155 AA; 17723 MW; 36879AAF1CCFB961 CRC64;
MGPKLLESRL CLLLLLGLVL MLASCQAQIL SQKFYTQHIY NSTYPRCDAV
MRVVNRYRPR CKDINTFLHT SFADVVAVCG HPNITCNNLT RKNCHASSFQ
VFITFCNLTM PTRICTQCRY QTTGSVKYYR VACENRTPQD TPMYPVVPVH
LDGTF

Mouse Rnase2 ELISA Kit

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