Mouse Pik3ca ELISA Kit

Alternative name
Mouse PI3-kinase subunit alpha ELISA Kit;Mouse PI3K-alpha ELISA Kit;Mouse PI3Kalpha ELISA Kit;Mouse ptdIns-3-kinase subunit alpha ELISA Kit;Mouse phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha ELISA Kit;Mouse ptdIns-3-kinase subunit p110-alpha ELISA Kit;Mouse p110alpha ELISA Kit;Mouse phosphoinositide-3-kinase catalytic alpha polypeptide ELISA Kit;Mouse serine/threonine protein kinase PIK3CA ELISA Kit;Mouse 6330412C24Rik ELISA Kit;Mouse caPI3K ELISA Kit;Mouse p110 ELISA Kit;Mouse phosphatidylinositol 3-kinase, catalytic, alpha polypeptide ELISA Kit;Mouse phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform ELISA Kit;
Catalog
E063488
species
Mouse
GenePik3ca
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.469ng/ml
Intended UseMouse Pik3ca ELISA Kit allows for the in vitro quantitative determination of Pik3ca , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse Pik3ca antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Pik3ca antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Pik3ca amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Pik3ca can be calculated.
Mouse Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform Protein information
Uniprot ID PK3CA_MOUSE
Uniprot AC P42337; Q0VGQ5;
UniGene Mm.260521;
GeneID 18706
KEGG mmu:18706;
Mouse Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform Protein SEQUENCE
SEQUENCE 1068 AA; 124412 MW; D593283B416ABFD0 CRC64;
MPPRPSSGEL WGIHLMPPRI LVECLLPNGM IVTLECLREA TLVTIKHELF
REARKYPLHQ LLQDETSYIF VSVTQEAERE EFFDETRRLC DLRLFQPFLK
VIEPVGNREE KILNREIGFV IGMPVCEFDM VKDPEVQDFR RNILNVCKEA
VDLRDLNSPH SRAMYVYPPN VESSPELPKH IYNKLDKGQI IVVIWVIVSP
NNDKQKYTLK INHDCVPEQV IAEAIRKKTR SMLLSSEQLK LCVLEYQGKY
ILKVCGCDEY FLEKYPLSQY KYIRSCIMLG RMPNLMLMAK ESLYSQLPID
SFTMPSYSRR ISTATPYMNG ETSTKSLWVI NSALRIKILC ATYVNVNIRD
IDKIYVRTGI YHGGEPLCDN VNTQRVPCSN PRWNEWLNYD IYIPDLPRAA
RLCLSICSVK GRKGAKEEHC PLAWGNINLF DYTDTLVSGK MALNLWPVPH
GLEDLLNPIG VTGSNPNKET PCLELEFDWF SSVVKFPDMS VIEEHANWSV
SREAGFSYSH TGLSNRLARD NELRENDKEQ LRALCTRDPL SEITEQEKDF
LWSHRHYCVT IPEILPKLLL SVKWNSRDEV AQMYCLVKDW PPIKPEQAME
LLDCNYPDPM VRSFAVRCLE KYLTDDKLSQ YLIQLVQVLK YEQYLDNLLV
RFLLKKALTN QRIGHFFFWH LKSEMHNKTV SQRFGLLLES YCRACGMYLK
HLNRQVEAME KLINLTDILK QEKKDETQKV QMKFLVEQMR QPDFMDALQG
FLSPLNPAHQ LGNLRLEECR IMSSAKRPLW LNWENPDIMS ELLFQNNEII
FKNGDDLRQD MLTLQIIRIM ENIWQNQGLD LRMLPYGCLS IGDCVGLIEV
VRNSHTIMQI QCKGGLKGAL QFNSHTLHQW LKDKNKGEIY DAAIDLFTRS
CAGYCVATFI LGIGDRHNSN IMVKDDGQLF HIDFGHFLDH KKKKFGYKRE
RVPFVLTQDF LIVISKGAQE YTKTREFERF QEMCYKAYLA IRQHANLFIN
LFSMMLGSGM PELQSFDDIA YIRKTLALDK TEQEALEYFT KQMNDAHHGG
WTTKMDWIFH TIKQHALN
UCSC uc008owd.2; mouse.;

Category

Mouse Pik3ca ELISA Kit