Human MAN2B1 ELISA Kit

Alternative name
Human LAMAN ELISA Kit;Human lysosomal acid alpha-mannosidase ELISA Kit;Human mannosidase alpha class 2B member 1 ELISA Kit;Human mannosidase alpha-B ELISA Kit;Human MANB ELISA Kit;Human lysosomal alpha-mannosidase ELISA Kit;Human mannosidase, alpha B, lysosomal ELISA Kit;
Catalog
E023626
species
Human
GeneMAN2B1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Mannosidase Alpha Class 2B Member 1 (MAN2B1). No significant cross-reactivity or interference between Mannosidase Alpha Class 2B Member 1 (MAN2B1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 14.7pg/mL.
Intended UseHuman MAN2B1 ELISA Kit allows for the in vitro quantitative determination of MAN2B1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mannosidase Alpha Class 2B Member 1 (MAN2B1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mannosidase Alpha Class 2B Member 1 (MAN2B1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mannosidase Alpha Class 2B Member 1 (MAN2B1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mannosidase Alpha Class 2B Member 1 (MAN2B1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Lysosomal alpha-mannosidase E peptide Protein information
Uniprot ID MA2B1_HUMAN
Uniprot AC O00754; G5E928; O15330; Q16680; Q93094; Q9BW13;
UniGene Hs.356769; Hs.657204;
GeneID 4125
KEGG hsa:4125;
Human Lysosomal alpha-mannosidase E peptide Protein SEQUENCE
SEQUENCE 1011 AA; 113744 MW; E11C77C19D8BD88C CRC64;
MGAYARASGV CARGCLDSAG PWTMSRALRP PLPPLCFFLL LLAAAGARAG
GYETCPTVQP NMLNVHLLPH THDDVGWLKT VDQYFYGIKN DIQHAGVQYI
LDSVISALLA DPTRRFIYVE IAFFSRWWHQ QTNATQEVVR DLVRQGRLEF
ANGGWVMNDE AATHYGAIVD QMTLGLRFLE DTFGNDGRPR VAWHIDPFGH
SREQASLFAQ MGFDGFFFGR LDYQDKWVRM QKLEMEQVWR ASTSLKPPTA
DLFTGVLPNG YNPPRNLCWD VLCVDQPLVE DPRSPEYNAK ELVDYFLNVA
TAQGRYYRTN HTVMTMGSDF QYENANMWFK NLDKLIRLVN AQQAKGSSVH
VLYSTPACYL WELNKANLTW SVKHDDFFPY ADGPHQFWTG YFSSRPALKR
YERLSYNFLQ VCNQLEALVG LAANVGPYGS GDSAPLNEAM AVLQHHDAVS
GTSRQHVAND YARQLAAGWG PCEVLLSNAL ARLRGFKDHF TFCQQLNISI
CPLSQTAARF QVIVYNPLGR KVNWMVRLPV SEGVFVVKDP NGRTVPSDVV
IFPSSDSQAH PPELLFSASL PALGFSTYSV AQVPRWKPQA RAPQPIPRRS
WSPALTIENE HIRATFDPDT GLLMEIMNMN QQLLLPVRQT FFWYNASIGD
NESDQASGAY IFRPNQQKPL PVSRWAQIHL VKTPLVQEVH QNFSAWCSQV
VRLYPGQRHL ELEWSVGPIP VGDTWGKEVI SRFDTPLETK GRFYTDSNGR
EILERRRDYR PTWKLNQTEP VAGNYYPVNT RIYITDGNMQ LTVLTDRSQG
GSSLRDGSLE LMVHRRLLKD DGRGVSEPLM ENGSGAWVRG RHLVLLDTAQ
AAAAGHRLLA EQEVLAPQVV LAPGGGAAYN LGAPPRTQFS GLRRDLPPSV
HLLTLASWGP EMVLLRLEHQ FAVGEDSGRN LSAPVTLNLR DLFSTFTITR
LQETTLVANQ LREAASRLKW TTNTGPTPHQ TPYQLDPANI TLEPMEIRTF
LASVQWKEVD G
UCSC uc002mub.3; human. [O00754-1];

Category

Human MAN2B1 ELISA Kit

Human LAMAN ELISA Kit;Human lysosomal acid alpha-mannosidase ELISA Kit;Human mannosidase alpha class 2B member 1 ELISA Kit;Human mannosidase alpha-B ELISA Kit;Human MANB ELISA Kit;Human lysosomal alpha-mannosidase ELISA Kit;Human mannosidase, alpha B, lysosomal ELISA Kit;