Human MAN2B1 ELISA Kit

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  • Alternative name

    Human LAMAN ELISA Kit;Human lysosomal acid alpha-mannosidase ELISA Kit;Human mannosidase alpha class 2B member 1 ELISA Kit;Human mannosidase alpha-B ELISA Kit;Human MANB ELISA Kit;Human lysosomal alpha-mannosidase ELISA Kit;Human mannosidase, alpha B, lysosomal ELISA Kit;

  • Catalog
    E023626
  • species
    Human
  • GeneMAN2B1
  • Standard CurveHuman MAN2B1 ELISA Kit
  • Other Species Mouse Man2b1 ELISA KitBovine MAN2B1 ELISA KitGuinea Pig MAN2B1 ELISA KitMonkey MAN2B1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mannosidase Alpha Class 2B Member 1 (MAN2B1). No significant cross-reactivity or interference between Mannosidase Alpha Class 2B Member 1 (MAN2B1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 14.7pg/mL.
  • Intended UseHuman MAN2B1 ELISA Kit allows for the in vitro quantitative determination of MAN2B1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mannosidase Alpha Class 2B Member 1 (MAN2B1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mannosidase Alpha Class 2B Member 1 (MAN2B1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mannosidase Alpha Class 2B Member 1 (MAN2B1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mannosidase Alpha Class 2B Member 1 (MAN2B1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Lysosomal alpha-mannosidase E peptide Protein information
  • Uniprot ID MA2B1_HUMAN
  • Uniprot AC O00754; G5E928; O15330; Q16680; Q93094; Q9BW13;
  • UniGene Hs.356769; Hs.657204;
  • GeneID 4125
  • KEGG hsa:4125;
  • Human Lysosomal alpha-mannosidase E peptide Protein SEQUENCE
  • SEQUENCE 1011 AA; 113744 MW; E11C77C19D8BD88C CRC64;

    MGAYARASGV CARGCLDSAG PWTMSRALRP PLPPLCFFLL LLAAAGARAG

    GYETCPTVQP NMLNVHLLPH THDDVGWLKT VDQYFYGIKN DIQHAGVQYI

    LDSVISALLA DPTRRFIYVE IAFFSRWWHQ QTNATQEVVR DLVRQGRLEF

    ANGGWVMNDE AATHYGAIVD QMTLGLRFLE DTFGNDGRPR VAWHIDPFGH

    SREQASLFAQ MGFDGFFFGR LDYQDKWVRM QKLEMEQVWR ASTSLKPPTA

    DLFTGVLPNG YNPPRNLCWD VLCVDQPLVE DPRSPEYNAK ELVDYFLNVA

    TAQGRYYRTN HTVMTMGSDF QYENANMWFK NLDKLIRLVN AQQAKGSSVH

    VLYSTPACYL WELNKANLTW SVKHDDFFPY ADGPHQFWTG YFSSRPALKR

    YERLSYNFLQ VCNQLEALVG LAANVGPYGS GDSAPLNEAM AVLQHHDAVS

    GTSRQHVAND YARQLAAGWG PCEVLLSNAL ARLRGFKDHF TFCQQLNISI

    CPLSQTAARF QVIVYNPLGR KVNWMVRLPV SEGVFVVKDP NGRTVPSDVV

    IFPSSDSQAH PPELLFSASL PALGFSTYSV AQVPRWKPQA RAPQPIPRRS

    WSPALTIENE HIRATFDPDT GLLMEIMNMN QQLLLPVRQT FFWYNASIGD

    NESDQASGAY IFRPNQQKPL PVSRWAQIHL VKTPLVQEVH QNFSAWCSQV

    VRLYPGQRHL ELEWSVGPIP VGDTWGKEVI SRFDTPLETK GRFYTDSNGR

    EILERRRDYR PTWKLNQTEP VAGNYYPVNT RIYITDGNMQ LTVLTDRSQG

    GSSLRDGSLE LMVHRRLLKD DGRGVSEPLM ENGSGAWVRG RHLVLLDTAQ

    AAAAGHRLLA EQEVLAPQVV LAPGGGAAYN LGAPPRTQFS GLRRDLPPSV

    HLLTLASWGP EMVLLRLEHQ FAVGEDSGRN LSAPVTLNLR DLFSTFTITR

    LQETTLVANQ LREAASRLKW TTNTGPTPHQ TPYQLDPANI TLEPMEIRTF

    LASVQWKEVD G

  • UCSC uc002mub.3; human. [O00754-1];



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