Mouse Pdgfb ELISA Kit

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  • Alternative name

    Mouse PDGF subunit B ELISA Kit;Mouse PDGF-2 ELISA Kit;Mouse platelet-derived growth factor B chain ELISA Kit;Mouse platelet-derived growth factor beta polypeptide ELISA Kit;Mouse proto-oncogene c-Sis ELISA Kit;Mouse Sis ELISA Kit;Mouse PDGF-B ELISA Kit;Mouse platelet derived growth factor, B polypeptide ELISA Kit;Mouse platelet-derived growth factor subunit B ELISA Kit;Mouse c-sis ELISA Kit;

  • Catalog
    E063082
  • species
    Mouse
  • GenePdgfb
  • Other Species Human PDGFB,PDGFA ELISA KitHuman PDGFB ELISA KitMouse PDGFB,PDGFA ELISA KitCanine PDGFB ELISA KitPorcine PDGFB ELISA KitRat Pdgfb ELISA KitSheep PDGFB ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse PDGFB. No significant cross-reactivity or interference between Mouse PDGFB and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse PDGFB and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity18.75pg/mL
  • Intended UseMouse Pdgfb ELISA Kit allows for the in vitro quantitative determination of Pdgfb , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PDGFB in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PDGFB. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse PDGFB and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PDGFB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse PDGFB. You can calculate the concentration of Mouse PDGFB in the samples by comparing the OD of the samples to the standard curve.
  • Mouse Platelet-derived growth factor subunit B Protein information
  • Uniprot ID PDGFB_MOUSE
  • Uniprot AC P31240; Q6P3C4;
  • UniGene Mm.144089;
  • GeneID 18591
  • KEGG mmu:18591;
  • Mouse Platelet-derived growth factor subunit B Protein SEQUENCE
  • SEQUENCE 241 AA; 27382 MW; 3C5EB7A2DAD64178 CRC64;

    MNRCWALFLP LCCYLRLVSA EGDPIPEELY EMLSDHSIRS FDDLQRLLHR

    DSVDEDGAEL DLNMTRAHSG VELESSSRGR RSLGSLAAAE PAVIAECKTR

    TEVFQISRNL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRASQVQMRP

    VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETIVTPRPVT RSPGTSREQR

    AKTPQARVTI RTVRIRRPPK GKHRKFKHTH DKAALKETLG A

  • UCSC uc007wuz.1; mouse.;



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