Human EED ELISA Kit

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  • Alternative name

    Human HEED ELISA Kit;Human WD protein associating with integrin cytoplasmic tails 1 ELISA Kit;Human WAIT-1 ELISA Kit;Human WAIT1 ELISA Kit;Human embryonic ectoderm development ELISA Kit;Human polycomb protein EED ELISA Kit;

  • Catalog
    E012769
  • species
    Human
  • GeneEED
  • Other Species Mouse Eed ELISA KitBovine EED ELISA KitChicken EED ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human EED. No significant cross-reactivity or interference between Human EED and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human EED and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman EED ELISA Kit allows for the in vitro quantitative determination of EED , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of EED in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human EED. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human EED and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human EED, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human EED. You can calculate the concentration of Human EED in the samples by comparing the OD of the samples to the standard curve.
  • Human Polycomb protein EED Protein information
  • Uniprot ID EED_HUMAN
  • Uniprot AC O75530; A8K7V5; O00149; Q6NTH2; Q7LDA5; Q7LDG8; Q86VV2; Q9UNY7;
  • UniGene Hs.503510; Hs.528103;
  • GeneID 8726
  • KEGG hsa:8726;
  • Human Polycomb protein EED Protein SEQUENCE
  • SEQUENCE 441 AA; 50198 MW; D2E0A5BA27C0499A CRC64;

    MSEREVSTAP AGTDMPAAKK QKLSSDENSN PDLSGDENDD AVSIESGTNT

    ERPDTPTNTP NAPGRKSWGK GKWKSKKCKY SFKCVNSLKE DHNQPLFGVQ

    FNWHSKEGDP LVFATVGSNR VTLYECHSQG EIRLLQSYVD ADADENFYTC

    AWTYDSNTSH PLLAVAGSRG IIRIINPITM QCIKHYVGHG NAINELKFHP

    RDPNLLLSVS KDHALRLWNI QTDTLVAIFG GVEGHRDEVL SADYDLLGEK

    IMSCGMDHSL KLWRINSKRM MNAIKESYDY NPNKTNRPFI SQKIHFPDFS

    TRDIHRNYVD CVRWLGDLIL SKSCENAIVC WKPGKMEDDI DKIKPSESNV

    TILGRFDYSQ CDIWYMRFSM DFWQKMLALG NQVGKLYVWD LEVEDPHKAK

    CTTLTHHKCG AAIRQTSFSR DSSILIAVCD DASIWRWDRL R

  • UCSC uc001pbp.4; human. [O75530-1];


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