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Mouse Pdap1 ELISA Kit

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  • Alternative name

    Mouse PDGF-associated protein ELISA Kit;Mouse PAP ELISA Kit;Mouse PDGFA-associated protein 1 ELISA Kit;Mouse PAP1 ELISA Kit;Mouse HASPP28 ELISA Kit;Mouse PDGFA associated protein 1 ELISA Kit;Mouse 28 kDa heat- and acid-stable phosphoprotein ELISA Kit;
  • Catalog
    E063071
  • species
    Mouse
  • GenePdap1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse PDAP1. No significant cross-reactivity or interference between Mouse PDAP1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse PDAP1 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseMouse Pdap1 ELISA Kit allows for the in vitro quantitative determination of Pdap1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PDAP1 in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PDAP1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse PDAP1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PDAP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse PDAP1. You can calculate the concentration of Mouse PDAP1 in the samples by comparing the OD of the samples to the standard curve.
  • Mouse 28 kDa heat- and acid-stable phosphoprotein Protein information
  • Uniprot ID HAP28_MOUSE
  • Uniprot AC Q3UHX2; A0JLS1; Q3KQJ5;
  • UniGene Mm.188851;
  • GeneID 231887
  • KEGG mmu:231887;
  • Mouse 28 kDa heat- and acid-stable phosphoprotein Protein SEQUENCE

  • SEQUENCE 181 AA; 20605 MW; C9A74B7C41174F87 CRC64;

    MPKGGRKGGH KGRVRQYTSP EEIDAQLQAE KQKANEEDEQ EEGGDGASGD

    PKKEKKSLDS DESEDEDDDY QQKRKGVEGL IDIENPNRVA QTTKKVTQLD

    LDGPKELSRR EREEIEKQKA KERYMKMHLA GKTEQAKADL ARLAIIRKQR

    EEAARKKEEE RKAKDDATLS GKRMQSLSLN K

  • UCSC uc009ame.1; mouse.;



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