Contact Us
  • Alternative name

    Icd ELISA KIT; Icd ELISA KIT; Icd proteinicd ELISA KIT;

  • Catalog
  • species
  • GeneICD
  • Other Species Mouse ICD ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman ICD ELISA Kit allows for the in vitro quantitative determination of ICD , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Principle of the Assay: ICD ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ICD antibody and an ICD-HRP conjugate. The assay sample and buffer are incubated together with ICD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ICD concentration since ICD from samples and ICD-HRP conjugate compete for the anti-ICD antibody binding site. Since the number of sites is limited, as more sites are occupied by ICD from the sample, fewer sites are left to bind ICD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ICD concentration in each sample is interpolated from this standard curve.

Related products with Human ICD ELISA Kit

Inquiry About Human ICD ELISA Kit If you hope to order it or contact us directly, please contact us via We will get back to you in 12hrs,Thanks!