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Alternative name
Chitinase-like protein 3 ELISA KIT; Beta-N-acetylhexosaminidase Ym1 ELISA KIT; Chitinase-3-like protein 3 ELISA KIT; ECF-L ELISA KIT; Eosinophil chemotactic cytokine ELISA KIT; Secreted protein Ym1Chil3 ELISA KIT; Chi3l3 ELISA KIT; Ym1 ELISA KIT
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Catalog
E048243
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species
Mouse
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GeneChi3l3
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Standard Curve
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SpecificityThis assay recognizes recombinant and natural mouse CHI3L3. No significant cross-reactivity or interference was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Intended UseMouse Chi3l3 ELISA Kit allows for the in vitro quantitative determination of Chi3l3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CHI3L3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CHI3L3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CHI3L3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of CHI3L3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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