Human PRKAR1A ELISA Kit

Alternative name
Human tissue-specific extinguisher 1 ELISA Kit;Human TSE1 ELISA Kit;Human PKR1 ELISA Kit;Human PRKAR1 ELISA Kit;Human ACRDYS1 ELISA Kit;Human ADOHR ELISA Kit;Human CAR ELISA Kit;Human CNC ELISA Kit;Human CNC1 ELISA Kit;Human PPNAD1 ELISA Kit;Human protein kinase cAMP-dependent type I regulatory subunit alpha ELISA Kit;Human cAMP-dependent protein kinase type I-alpha regulatory subunit ELISA Kit;Human Carney complex type 1 ELISA Kit;Human cAMP-dependent protein kinase regulatory subunit RIalpha ELISA Kit;Human cAMP-dependent protein kinase type I-alpha regulatory chain ELISA Kit;Human protein kinase A type 1a regulatory subunit ELISA Kit;Human protein kinase, cAMP-dependent, regulatory subunit type I alpha ELISA Kit;Human protein kinase, cAMP-dependent, regulatory, type I, alpha ELISA Kit;
Catalog
E006294
species
Human
GenePRKAR1A
Standard Curve
Other Species Mouse Prkar1a ELISA Kit Bovine PRKAR1A ELISA Kit Chicken PRKAR1A ELISA Kit Porcine PRKAR1A ELISA Kit Rat Prkar1a ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of PRKAR1A. No significant cross-reactivity or interference between PRKAR1A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PRKAR1A and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman PRKAR1A ELISA Kit allows for the in vitro quantitative determination of PRKAR1A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the assay: PRKAR1A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PRKAR1A antibody and an PRKAR1A-HRP conjugate. The assay sample and buffer are incubated together with PRKAR1A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PRKAR1A concentration since PRKAR1A from samples and PRKAR1A-HRP conjugate compete for the anti-PRKAR1A antibody binding site. Since the number of sites is limited, as more sites are occupied by PRKAR1A from the sample, fewer sites are left to bind PRKAR1A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PRKAR1A concentration in each sample is interpolated from this standard curve.
Human cAMP-dependent protein kinase type I-alpha regulatory subunit, N-terminally processed Protein information
Uniprot ID KAP0_HUMAN
Uniprot AC P10644; K7ER48; Q567S7;
UniGene Hs.280342; Hs.745160;
GeneID 5573
KEGG hsa:5573;
Human cAMP-dependent protein kinase type I-alpha regulatory subunit, N-terminally processed Protein SEQUENCE
SEQUENCE 381 AA; 42982 MW; 2D04F08CE8857A6D CRC64;
MESGSTAASE EARSLRECEL YVQKHNIQAL LKDSIVQLCT ARPERPMAFL
REYFERLEKE EAKQIQNLQK AGTRTDSRED EISPPPPNPV VKGRRRRGAI
SAEVYTEEDA ASYVRKVIPK DYKTMAALAK AIEKNVLFSH LDDNERSDIF
DAMFSVSFIA GETVIQQGDE GDNFYVIDQG ETDVYVNNEW ATSVGEGGSF
GELALIYGTP RAATVKAKTN VKLWGIDRDS YRRILMGSTL RKRKMYEEFL
SKVSILESLD KWERLTVADA LEPVQFEDGQ KIVVQGEPGD EFFIILEGSA
AVLQRRSENE EFVEVGRLGP SDYFGEIALL MNRPRAATVV ARGPLKCVKL
DRPRFERVLG PCSDILKRNI QQYNSFVSLS V
UCSC uc002jhg.5; human. [P10644-1];

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Human PRKAR1A ELISA Kit