Human PRKAR1A ELISA Kit

Contact Us
  • Alternative name

    Human tissue-specific extinguisher 1 ELISA Kit;Human TSE1 ELISA Kit;Human PKR1 ELISA Kit;Human PRKAR1 ELISA Kit;Human ACRDYS1 ELISA Kit;Human ADOHR ELISA Kit;Human CAR ELISA Kit;Human CNC ELISA Kit;Human CNC1 ELISA Kit;Human PPNAD1 ELISA Kit;Human protein kinase cAMP-dependent type I regulatory subunit alpha ELISA Kit;Human cAMP-dependent protein kinase type I-alpha regulatory subunit ELISA Kit;Human Carney complex type 1 ELISA Kit;Human cAMP-dependent protein kinase regulatory subunit RIalpha ELISA Kit;Human cAMP-dependent protein kinase type I-alpha regulatory chain ELISA Kit;Human protein kinase A type 1a regulatory subunit ELISA Kit;Human protein kinase, cAMP-dependent, regulatory subunit type I alpha ELISA Kit;Human protein kinase, cAMP-dependent, regulatory, type I, alpha ELISA Kit;

  • Catalog
    E006294
  • species
    Human
  • GenePRKAR1A
  • Standard CurveHuman PRKAR1A ELISA Kit
  • Other Species Mouse Prkar1a ELISA KitBovine PRKAR1A ELISA KitChicken PRKAR1A ELISA KitPorcine PRKAR1A ELISA KitRat Prkar1a ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of PRKAR1A. No significant cross-reactivity or interference between PRKAR1A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PRKAR1A and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman PRKAR1A ELISA Kit allows for the in vitro quantitative determination of PRKAR1A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Principle of the assay: PRKAR1A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PRKAR1A antibody and an PRKAR1A-HRP conjugate. The assay sample and buffer are incubated together with PRKAR1A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PRKAR1A concentration since PRKAR1A from samples and PRKAR1A-HRP conjugate compete for the anti-PRKAR1A antibody binding site. Since the number of sites is limited, as more sites are occupied by PRKAR1A from the sample, fewer sites are left to bind PRKAR1A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PRKAR1A concentration in each sample is interpolated from this standard curve.
  • Human cAMP-dependent protein kinase type I-alpha regulatory subunit, N-terminally processed Protein information
  • Uniprot ID KAP0_HUMAN
  • Uniprot AC P10644; K7ER48; Q567S7;
  • UniGene Hs.280342; Hs.745160;
  • GeneID 5573
  • KEGG hsa:5573;
  • Human cAMP-dependent protein kinase type I-alpha regulatory subunit, N-terminally processed Protein SEQUENCE
  • SEQUENCE 381 AA; 42982 MW; 2D04F08CE8857A6D CRC64;

    MESGSTAASE EARSLRECEL YVQKHNIQAL LKDSIVQLCT ARPERPMAFL

    REYFERLEKE EAKQIQNLQK AGTRTDSRED EISPPPPNPV VKGRRRRGAI

    SAEVYTEEDA ASYVRKVIPK DYKTMAALAK AIEKNVLFSH LDDNERSDIF

    DAMFSVSFIA GETVIQQGDE GDNFYVIDQG ETDVYVNNEW ATSVGEGGSF

    GELALIYGTP RAATVKAKTN VKLWGIDRDS YRRILMGSTL RKRKMYEEFL

    SKVSILESLD KWERLTVADA LEPVQFEDGQ KIVVQGEPGD EFFIILEGSA

    AVLQRRSENE EFVEVGRLGP SDYFGEIALL MNRPRAATVV ARGPLKCVKL

    DRPRFERVLG PCSDILKRNI QQYNSFVSLS V

  • UCSC uc002jhg.5; human. [P10644-1];


Related products with Human PRKAR1A ELISA Kit


tag

Inquiry About Human PRKAR1A ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!