Humanman SAP ELISA Kit

Contact Us
  • Alternative name

    SAP ELISA KIT; SAPsap ELISA KIT;

  • Catalog
    E034221
  • species
    Humanman
  • GeneSAP
  • Other Species Human SAP ELISA KitMouse SAP ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHumanman SAP ELISA Kit allows for the in vitro quantitative determination of SAP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Serum amyloid P present in samples reacts with the anti-Serum amyloid P antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-SAP antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound SAP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromo-genic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of SAP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of SAP in the test sample. The quantity of SAP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.


Related products with Humanman SAP ELISA Kit

Inquiry About Humanman SAP ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!