Mouse Ncl ELISA Kit

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  • Alternative name

    Mouse Protein C23 ELISA Kit;Mouse Nuc ELISA Kit;Mouse B530004O11Rik ELISA Kit;Mouse C23 ELISA Kit;Mouse D0Nds28 ELISA Kit;Mouse D1Nds28 ELISA Kit;Mouse Nucl ELISA Kit;Mouse nucleolin ELISA Kit;

  • Catalog
    E062006
  • species
    Mouse
  • GeneNcl
  • Standard CurveMouse Ncl ELISA Kit
  • Other Species Human NCL ELISA KitChicken NCL ELISA KitMonkey NCL ELISA KitRat Ncl ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of nucleolin. No significant cross-reactivity or interference between nucleolin and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of nucleolin is typically less than 0.059ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by addin
  • Intended UseMouse Ncl ELISA Kit allows for the in vitro quantitative determination of Ncl , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to nucleolin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to nucleolin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain nucleolin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of nucleolin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse Nucleolin Protein information
  • Uniprot ID NUCL_MOUSE
  • Uniprot AC P09405; Q548M9; Q61991; Q8BQD8; Q99K50;
  • UniGene Mm.154378; Mm.474153;
  • GeneID 17975
  • KEGG mmu:17975;
  • Mouse Nucleolin Protein SEQUENCE
  • SEQUENCE 707 AA; 76723 MW; EE2CE2ACDBF54CD4 CRC64;

    MVKLAKAGKT HGEAKKMAPP PKEVEEDSED EEMSEDEDDS SGEEEVVIPQ

    KKGKKATTTP AKKVVVSQTK KAAVPTPAKK AAVTPGKKAV ATPAKKNITP

    AKVIPTPGKK GAAQAKALVP TPGKKGAATP AKGAKNGKNA KKEDSDEDED

    EEDEDDSDED EDDEEEDEFE PPIVKGVKPA KAAPAAPASE DEEDDEDEDD

    EEDDDEEEED DSEEEVMEIT TAKGKKTPAK VVPMKAKSVA EEEDDEEEDE

    DDEDEDDEEE DDEDDDEEEE EEEPVKAAPG KRKKEMTKQK EAPEAKKQKV

    EGSEPTTPFN LFIGNLNPNK SVNELKFAIS ELFAKNDLAV VDVRTGTNRK

    FGYVDFESAE DLEKALELTG LKVFGNEIKL EKPKGRDSKK VRAARTLLAK

    NLSFNITEDE LKEVFEDAME IRLVSQDGKS KGIAYIEFKS EADAEKNLEE

    KQGAEIDGRS VSLYYTGEKG QRQERTGKTS TWSGESKTLV LSNLSYSATK

    ETLEEVFEKA TFIKVPQNPH GKPKGYAFIE FASFEDAKEA LNSCNKMEIE

    GRTIRLELQG SNSRSQPSKT LFVKGLSEDT TEETLKESFE GSVRARIVTD

    RETGSSKGFG FVDFNSEEDA KAAKEAMEDG EIDGNKVTLD WAKPKGEGGF

    GGRGGGRGGF GGRGGGRGGR GGFGGRGRGG FGGRGGFRGG RGGGGDFKPQ

    GKKTKFE

  • UCSC uc007bvl.1; mouse.;


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