Mouse Ncl ELISA Kit

Alternative name
Mouse Protein C23 ELISA Kit;Mouse Nuc ELISA Kit;Mouse B530004O11Rik ELISA Kit;Mouse C23 ELISA Kit;Mouse D0Nds28 ELISA Kit;Mouse D1Nds28 ELISA Kit;Mouse Nucl ELISA Kit;Mouse nucleolin ELISA Kit;
Catalog
E062006
species
Mouse
GeneNcl
SpecificityThis assay has high sensitivity and excellent specificity for detection of nucleolin. No significant cross-reactivity or interference between nucleolin and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of nucleolin is typically less than 0.059ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by addin
Intended UseMouse Ncl ELISA Kit allows for the in vitro quantitative determination of Ncl , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to nucleolin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to nucleolin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain nucleolin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of nucleolin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse Nucleolin Protein information
Uniprot ID NUCL_MOUSE
Uniprot AC P09405; Q548M9; Q61991; Q8BQD8; Q99K50;
UniGene Mm.154378; Mm.474153;
GeneID 17975
KEGG mmu:17975;
Mouse Nucleolin Protein SEQUENCE
SEQUENCE 707 AA; 76723 MW; EE2CE2ACDBF54CD4 CRC64;
MVKLAKAGKT HGEAKKMAPP PKEVEEDSED EEMSEDEDDS SGEEEVVIPQ
KKGKKATTTP AKKVVVSQTK KAAVPTPAKK AAVTPGKKAV ATPAKKNITP
AKVIPTPGKK GAAQAKALVP TPGKKGAATP AKGAKNGKNA KKEDSDEDED
EEDEDDSDED EDDEEEDEFE PPIVKGVKPA KAAPAAPASE DEEDDEDEDD
EEDDDEEEED DSEEEVMEIT TAKGKKTPAK VVPMKAKSVA EEEDDEEEDE
DDEDEDDEEE DDEDDDEEEE EEEPVKAAPG KRKKEMTKQK EAPEAKKQKV
EGSEPTTPFN LFIGNLNPNK SVNELKFAIS ELFAKNDLAV VDVRTGTNRK
FGYVDFESAE DLEKALELTG LKVFGNEIKL EKPKGRDSKK VRAARTLLAK
NLSFNITEDE LKEVFEDAME IRLVSQDGKS KGIAYIEFKS EADAEKNLEE
KQGAEIDGRS VSLYYTGEKG QRQERTGKTS TWSGESKTLV LSNLSYSATK
ETLEEVFEKA TFIKVPQNPH GKPKGYAFIE FASFEDAKEA LNSCNKMEIE
GRTIRLELQG SNSRSQPSKT LFVKGLSEDT TEETLKESFE GSVRARIVTD
RETGSSKGFG FVDFNSEEDA KAAKEAMEDG EIDGNKVTLD WAKPKGEGGF
GGRGGGRGGF GGRGGGRGGR GGFGGRGRGG FGGRGGFRGG RGGGGDFKPQ
GKKTKFE
UCSC uc007bvl.1; mouse.;

Category

Mouse Ncl ELISA Kit

Mouse Protein C23 ELISA Kit;Mouse Nuc ELISA Kit;Mouse B530004O11Rik ELISA Kit;Mouse C23 ELISA Kit;Mouse D0Nds28 ELISA Kit;Mouse D1Nds28 ELISA Kit;Mouse Nucl ELISA Kit;Mouse nucleolin ELISA Kit;