Mouse Nucb1 ELISA Kit

Alternative name
Mouse Calnuc ELISA Kit;Mouse Nuc ELISA Kit;Mouse Nucb ELISA Kit;Mouse B230337F23Rik ELISA Kit;Mouse C77483 ELISA Kit;Mouse MTEST82 ELISA Kit;Mouse nucleobindin 1 ELISA Kit;Mouse nucleobindin-1 ELISA Kit;
Catalog
E061986
species
Mouse
GeneNucb1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Nucleobindin 1 (NUCB1). No significant cross-reactivity or interference between Nucleobindin 1 (NUCB1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.059ng/mL.
Intended UseMouse Nucb1 ELISA Kit allows for the in vitro quantitative determination of Nucb1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nucleobindin 1 (NUCB1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nucleobindin 1 (NUCB1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nucleobindin 1 (NUCB1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nucleobindin 1 (NUCB1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse Nucleobindin-1 Protein information
Uniprot ID NUCB1_MOUSE
Uniprot AC Q02819; Q3TU42; Q9CWA1; Q9D8J4; Q9DBL3;
UniGene Mm.258923;
GeneID 18220
KEGG mmu:18220;
Mouse Nucleobindin-1 Protein SEQUENCE
SEQUENCE 459 AA; 53409 MW; 1CC1102D216CC60B CRC64;
MPTSVPRGAP FLLLPPLLML SAVLAVPVDR AAPPQEDSQA TETPDTGLYY
HRYLQEVINV LETDGHFREK LQAANAEDIK SGKLSQELDF VSHNVRTKLD
ELKRQEVSRL RMLLKAKMDA KQEPNLQVDH MNLLKQFEHL DPQNQHTFEA
RDLELLIQTA TRDLAQYDAA HHEEFKRYEM LKEHERRRYL ESLGEEQRKE
AERKLQEQQR RHREHPKVNV PGSQAQLKEV WEELDGLDPN RFNPKTFFIL
HDINSDGVLD EQELEALFTK ELEKVYDPKN EEDDMREMEE ERLRMREHVM
KNVDTNQDRL VTLEEFLAST QRKEFGDTGE GWKTVEMSPA YTEEELKRFE
EELAAREAEL NARAQRLSQE TEALGRSQDR LEAQKRELQQ AVLQMEQRKQ
QLQEQSAPPS KPDGQLQFRA DTDDAPVPAP AGDQKDVPAS EKKVPEQPPE
LPQLDSQHL
UCSC uc009gvm.2; mouse.;

Category

Mouse Nucb1 ELISA Kit

Mouse Calnuc ELISA Kit;Mouse Nuc ELISA Kit;Mouse Nucb ELISA Kit;Mouse B230337F23Rik ELISA Kit;Mouse C77483 ELISA Kit;Mouse MTEST82 ELISA Kit;Mouse nucleobindin 1 ELISA Kit;Mouse nucleobindin-1 ELISA Kit;