Mouse Mtnd5 ELISA Kit

Alternative name
Mouse NADH dehydrogenase subunit 5 ELISA Kit;Mouse mt-Nd5 ELISA Kit;Mouse Nd5 ELISA Kit;
Catalog
E061126
species
Mouse
GeneMtnd5
SpecificityThis assay recognizes recombinant and natural mouse NADH-ubiquinone oxidoreductase chain 5. No significant cross-reactivity or interference was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseMouse Mtnd5 ELISA Kit allows for the in vitro quantitative determination of Mtnd5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NADH-ubiquinone oxidoreductase chain 5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for NADH-ubiquinone oxidoreductase chain 5 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NADH-ubiquinone oxidoreductase chain 5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of NADH-ubiquinone oxidoreductase chain 5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse NADH-ubiquinone oxidoreductase chain 5 Protein information
Uniprot ID NU5M_MOUSE
Uniprot AC P03921;
GeneID 17721
KEGG mmu:17721;
Mouse NADH-ubiquinone oxidoreductase chain 5 Protein SEQUENCE
SEQUENCE 607 AA; 68475 MW; 31C10862CDC78B10 CRC64;
MNIFTTSILL IFILLLSPIL ISMSNLIKHI NFPLYTTTSI KFSFIISLLP
LLMFFHNNME YMITTWHWVT MNSMELKMSF KTDFFSILFT SVALFVTWSI
MQFSSWYMHS DPNINRFIKY LTLFLITMLI LTSANNMFQL FIGWEGVGIM
SFLLIGWWYG RTDANTAALQ AILYNRIGDI GFILAMVWFS LNMNSWELQQ
IMFSNNNDNL IPLMGLLIAA TGKSAQFGLH PWLPSAMEGP TPVSALLHSS
TMVVAGIFLL VRFHPLTTNN NFILTTMLCL GALTTLFTAI CALTQNDIKK
IIAFSTSSQL GLMMVTLGMN QPHLAFLHIC THAFFKAMLF MCSGSIIHSL
ADEQDIRKMG NITKIMPFTS SCLVIGSLAL TGMPFLTGFY SKDLIIEAIN
TCNTNAWALL ITLIATSMTA MYSMRIIYFV TMTKPRFPPL ISINENDPDL
MNPIKRLAFG SIFAGFVISY NIPPTSIPVL TMPWFLKTTA LIISVLGFLI
ALELNNLTMK LSMNKANPYS SFSTLLGFFP SIIHRITPMK SLNLSLKTSL
TLLDLIWLEK TIPKSTSTLH TNMTTLTTNQ KGLIKLYFMS FLINIILIII
LYSINLE

Category

Mouse Mtnd5 ELISA Kit

Mouse NADH dehydrogenase subunit 5 ELISA Kit;Mouse mt-Nd5 ELISA Kit;Mouse Nd5 ELISA Kit;