Mouse Mtnd5 ELISA Kit

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  • Alternative name

    Mouse NADH dehydrogenase subunit 5 ELISA Kit;Mouse mt-Nd5 ELISA Kit;Mouse Nd5 ELISA Kit;

  • Catalog
    E061126
  • species
    Mouse
  • GeneMtnd5
  • Standard CurveMouse Mtnd5 ELISA Kit
  • Other Species Rat Mtnd5 ELISA Kit
  • SpecificityThis assay recognizes recombinant and natural mouse NADH-ubiquinone oxidoreductase chain 5. No significant cross-reactivity or interference was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseMouse Mtnd5 ELISA Kit allows for the in vitro quantitative determination of Mtnd5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NADH-ubiquinone oxidoreductase chain 5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for NADH-ubiquinone oxidoreductase chain 5 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NADH-ubiquinone oxidoreductase chain 5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of NADH-ubiquinone oxidoreductase chain 5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse NADH-ubiquinone oxidoreductase chain 5 Protein information
  • Uniprot ID NU5M_MOUSE
  • Uniprot AC P03921;
  • GeneID 17721
  • KEGG mmu:17721;
  • Mouse NADH-ubiquinone oxidoreductase chain 5 Protein SEQUENCE
  • SEQUENCE 607 AA; 68475 MW; 31C10862CDC78B10 CRC64;

    MNIFTTSILL IFILLLSPIL ISMSNLIKHI NFPLYTTTSI KFSFIISLLP

    LLMFFHNNME YMITTWHWVT MNSMELKMSF KTDFFSILFT SVALFVTWSI

    MQFSSWYMHS DPNINRFIKY LTLFLITMLI LTSANNMFQL FIGWEGVGIM

    SFLLIGWWYG RTDANTAALQ AILYNRIGDI GFILAMVWFS LNMNSWELQQ

    IMFSNNNDNL IPLMGLLIAA TGKSAQFGLH PWLPSAMEGP TPVSALLHSS

    TMVVAGIFLL VRFHPLTTNN NFILTTMLCL GALTTLFTAI CALTQNDIKK

    IIAFSTSSQL GLMMVTLGMN QPHLAFLHIC THAFFKAMLF MCSGSIIHSL

    ADEQDIRKMG NITKIMPFTS SCLVIGSLAL TGMPFLTGFY SKDLIIEAIN

    TCNTNAWALL ITLIATSMTA MYSMRIIYFV TMTKPRFPPL ISINENDPDL

    MNPIKRLAFG SIFAGFVISY NIPPTSIPVL TMPWFLKTTA LIISVLGFLI

    ALELNNLTMK LSMNKANPYS SFSTLLGFFP SIIHRITPMK SLNLSLKTSL

    TLLDLIWLEK TIPKSTSTLH TNMTTLTTNQ KGLIKLYFMS FLINIILIII

    LYSINLE





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