Intended UseMouse DHEA-S ELISA Kit allows for the in vitro quantitative determination of DHEA-S , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for DHEA-S and Horseradish Peroxidase (HRP) conjugated DHEA-S. The competitive inhibition reaction is launched between with HRP labeled DHEA-S and unlabeled DHEA-S with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of DHEA-S in the sample. The color development is stopped and the intensity of the color is measured.