Mouse Mttp ELISA Kit

Alternative name
Mouse MTP ELISA Kit;Mouse 1810043K16Rik ELISA Kit;Mouse microsomal triglyceride transfer protein ELISA Kit;Mouse microsomal triglyceride transfer protein large subunit ELISA Kit;Mouse microsomal triglyceride transfer protein B ELISA Kit;Mouse microsomal triglyceride transfer protein, large subunit ELISA Kit;
Catalog
E060094
species
Mouse
GeneMttp
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseMouse Mttp ELISA Kit allows for the in vitro quantitative determination of Mttp , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntended Uses: This MTTP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse MTTP. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: MTTP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MTTP antibody and an MTTP-HRP conjugate. The assay sample and buffer are incubated together with MTTP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MTTP concentration since MTTP from samples and MTTP-HRP conjugate compete for the anti-MTTP antibody binding site. Since the number of sites is limited, as more sites are occupied by MTTP from the sample, fewer sites are left to bind MTTP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MTTP concentration in each sample is interpolated from this standard curve.
Mouse Microsomal triglyceride transfer protein large subunit Protein information
Uniprot ID MTP_MOUSE
Uniprot AC O08601; B2CXA7; Q3UJA0; Q91X33;
UniGene Mm.2941;
GeneID 17777
KEGG mmu:17777;
Mouse Microsomal triglyceride transfer protein large subunit Protein SEQUENCE
SEQUENCE 894 AA; 99099 MW; 3D8E5EB470CAA6FC CRC64;
MILLAVLFLC FFSSYSASVK GHTTGLSLNN ERLYKLTYST EVFLDGGKGK
PQDSVGYKIS SDVDVVLLWR NPDGDDDQVI QVTITAVNVE NAGQQRGEKS
IFQGKSTPKI IGKDNLEALQ RPMLLHLVRG KVKEFYSYEN EPVGIENLKR
GLASLFQMQL SSGTTNEVDI SGDCKVTYQA QQDKVVKIKA LDTCKIERSG
FTTANQVLGV SSKATSVTTY KIEDSFVTAV LAEETRAFAL NFQQTIAGKI
VSKQKLELKT TEAGPRMIPG KQVAGVIKAV DSKYKAIPIV GQVLERVCKG
CPSLAEHWKS IRKNLEPENL SKAEAVQSFL AFIQHLRTSR REEILQILKA
EKKEVLPQLV DAVTSAQTPD SLEAILDFLD FKSDSSIILQ ERFLYACGFA
THPDEELLRA LLSKFKGSFA SNDIRESVMI IIGALVRKLC QNEGCKLKAV
VEAKKLILGG LEKPEKKEDT TMYLLALKNA LLPEGIPLLL KYAEAGEGPV
SHLATTVLQR YDVSFITDEV KKTLNRIYHQ NRKVHEKTVR TTAAAVILKN
PSYMDVKNIL LSIGELPKEM NKYMLTVVQD ILHFEMPASK MIRRVLKEMA
VHNYDRFSKS GSSSAYTGYV ERSPRAASTY SLDILYSGSG ILRRSNLNIF
QYIGKAELHG SQVVIEAQGL EGLIAATPDE GEENLDSYAG MSAILFDVQL
RPVTFFNGYS DLMSKMLSAS GDPVSVVKGL ILLIDHSQDI QLQSGLKANM
EIQGGLAIDI SGSMEFSLWY RESKTRVKNR VAVVITSDVT VDASFVKAGV
ESRAETEAGL EFISTVQFSQ YPFLVCMQMD KAEAPLRQFE TKYERLSTGR
GYVSRRRKES LVAGCELPLH QENSEMCNVV FPPQPESDNS GGWF
UCSC uc008rmx.2; mouse. [O08601-2];

Category

Mouse Mttp ELISA Kit