Human CENPB ELISA Kit

Alternative name
Human centromere protein B ELISA Kit;Human CENP-B ELISA Kit;Human major centromere autoantigen B ELISA Kit;Human centromere autoantigen B ELISA Kit;Human centromere protein B, 80kDa ELISA Kit;
Catalog
E007768
species
Human
GeneCENPB
SpecificityThis assay has high sensitivity and excellent specificity for detection of human CENP-B. No significant cross-reactivity or interference between human CENP-B and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human CENP-B is typically less than 23.4 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the m
Intended UseHuman CENPB ELISA Kit allows for the in vitro quantitative determination of CENPB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CENP-B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CENP-B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CENP-B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CENP-B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Major centromere autoantigen B Protein information
Uniprot ID CENPB_HUMAN
Uniprot AC P07199; Q96EI4;
UniGene Hs.516855;
GeneID 1059
KEGG hsa:1059;
Human Major centromere autoantigen B Protein SEQUENCE
SEQUENCE 599 AA; 65171 MW; 9B4B7DB957A914AA CRC64;
MGPKRRQLTF REKSRIIQEV EENPDLRKGE IARRFNIPPS TLSTILKNKR
AILASERKYG VASTCRKTNK LSPYDKLEGL LIAWFQQIRA AGLPVKGIIL
KEKALRIAEE LGMDDFTASN GWLDRFRRRH GVVSCSGVAR ARARNAAPRT
PAAPASPAAV PSEGSGGSTT GWRAREEQPP SVAEGYASQD VFSATETSLW
YDFLPDQAAG LCGGDGRPRQ ATQRLSVLLC ANADGSEKLP PLVAGKSAKP
RAGQAGLPCD YTANSKGGVT TQALAKYLKA LDTRMAAESR RVLLLAGRLA
AQSLDTSGLR HVQLAFFPPG TVHPLERGVV QQVKGHYRQA MLLKAMAALE
GQDPSGLQLG LTEALHFVAA AWQAVEPSDI AACFREAGFG GGPNATITTS
LKSEGEEEEE EEEEEEEEEG EGEEEEEEGE EEEEEGGEGE ELGEEEEVEE
EGDVDSDEEE EEDEESSSEG LEAEDWAQGV VEAGGSFGAY GAQEEAQCPT
LHFLEGGEDS DSDSEEEDDE EEDDEDEDDD DDEEDGDEVP VPSFGEAMAY
FAMVKRYLTS FPIDDRVQSH ILHLEHDLVH VTRKNHARQA GVRGLGHQS
UCSC uc002wjk.3; human.;

Category

Human CENPB ELISA Kit

Human centromere protein B ELISA Kit;Human CENP-B ELISA Kit;Human major centromere autoantigen B ELISA Kit;Human centromere autoantigen B ELISA Kit;Human centromere protein B, 80kDa ELISA Kit;