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  • GeneMSH
  • Standard CurveMouse MSH ELISA Kit
  • Other Species Human alpha-MSH ELISA KitHuman beta-MSH ELISA KitHuman MSH ELISA KitMouse alpha-MSH ELISA KitMouse beta-MSH ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseMouse MSH ELISA Kit allows for the in vitro quantitative determination of MSH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilySignal Transduction
  • Product Description
    For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate INTENDED USE This MSH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse MSH. This ELISA kit for research use only, not for therapeutic or diagnostic applications! PRINCIPLE OF THE ASSAY MSH ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MSH antibody and an MSH-HRP conjugate. The assay sample and buffer are incubated together with MSH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MSH concentration since MSH from samples and MSH-HRP conjugate compete for the anti-MSH antibody binding site. Since the number of sites is limited, as more sites are occupied by MSH from the sample, fewer sites are left to bind MSH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MSH concentration in each sample is interpolated from this standard curve.

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