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Alternative name
Matrix metalloproteinase 1, isoform C ELISA KIT; Matrix metalloproteinase 1, isoform CMmp1 ELISA KIT;
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Catalog
E059582
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species
Mouse
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GeneMMP1
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SpecificityThis assay has high sensitivity and excellent specificity for detection of MMP1. No significant cross-reactivity or interference between MMP1 and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity24.8pg/mL.
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Intended UseMouse MMP1 ELISA Kit allows for the in vitro quantitative determination of MMP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalIntended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MMP1 in mouse serum, plasma and other biological fluids.
Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to MMP1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to MMP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MMP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MMP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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