Human CDH16 ELISA Kit

Alternative name
Human kidney-specific cadherin ELISA Kit;Human KSP-cadherin ELISA Kit;Human cadherin 16 ELISA Kit;Human cadherin-16 ELISA Kit;Human cadherin 16, KSP-cadherin ELISA Kit;
Catalog
E005839
species
Human
GeneCDH16
Standard Curve
Other Species Mouse Cdh16 ELISA Kit Rabbit CDH16 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of CDH16. No significant cross-reactivity or interference between CDH16 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CDH16 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman CDH16 ELISA Kit allows for the in vitro quantitative determination of CDH16 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalIntended Uses: This CDH16 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CDH16. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||CDH16 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-CDH16 antibody and an CDH16-HRP conjugate. The assay sample and buffer are incubated together with CDH16-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CDH16 concentration since CDH16 from samples and CDH16-HRP conjugate compete for the anti-CDH16 antibody binding site. Since the number of sites is limited, as more sites are occupied by CDH16 from the sample, fewer sites are left to bind CDH16-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CDH16 concentration in each sample is interpolated from this standard curve.
Human Cadherin-16 Protein information
Uniprot ID CAD16_HUMAN
Uniprot AC O75309; B4DPA8; H3BPD3; Q6UW93;
UniGene Hs.513660;
GeneID 1014
KEGG hsa:1014;
Human Cadherin-16 Protein SEQUENCE
SEQUENCE 829 AA; 89923 MW; 41966915E4BFC891 CRC64;
MVPAWLWLLC VSVPQALPKA QPAELSVEVP ENYGGNFPLY LTKLPLPREG
AEGQIVLSGD SGKATEGPFA MDPDSGFLLV TRALDREEQA EYQLQVTLEM
QDGHVLWGPQ PVLVHVKDEN DQVPHFSQAI YRARLSRGTR PGIPFLFLEA
SDRDEPGTAN SDLRFHILSQ APAQPSPDMF QLEPRLGALA LSPKGSTSLD
HALERTYQLL VQVKDMGDQA SGHQATATVE VSIIESTWVS LEPIHLAENL
KVLYPHHMAQ VHWSGGDVHY HLESHPPGPF EVNAEGNLYV TRELDREAQA
EYLLQVRAQN SHGEDYAAPL ELHVLVMDEN DNVPICPPRD PTVSIPELSP
PGTEVTRLSA EDADAPGSPN SHVVYQLLSP EPEDGVEGRA FQVDPTSGSV
TLGVLPLRAG QNILLLVLAM DLAGAEGGFS STCEVEVAVT DINDHAPEFI
TSQIGPISLP EDVEPGTLVA MLTAIDADLE PAFRLMDFAI ERGDTEGTFG
LDWEPDSGHV RLRLCKNLSY EAAPSHEVVV VVQSVAKLVG PGPGPGATAT
VTVLVERVMP PPKLDQESYE ASVPISAPAG SFLLTIQPSD PISRTLRFSL
VNDSEGWLCI EKFSGEVHTA QSLQGAQPGD TYTVLVEAQD TDEPRLSASA
PLVIHFLKAP PAPALTLAPV PSQYLCTPRQ DHGLIVSGPS KDPDLASGHG
PYSFTLGPNP TVQRDWRLQT LNGSHAYLTL ALHWVEPREH IIPVVVSHNA
QMWQLLVRVI VCRCNVEGQC MRKVGRMKGM PTKLSAVGIL VGTLVAIGIF
LILIFTHWTM SRKKDPDQPA DSVPLKATV
UCSC uc002eql.3; human. [O75309-1];

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Category

Human CDH16 ELISA Kit

Human kidney-specific cadherin ELISA Kit;Human KSP-cadherin ELISA Kit;Human cadherin 16 ELISA Kit;Human cadherin-16 ELISA Kit;Human cadherin 16, KSP-cadherin ELISA Kit;