Human CDH1 ELISA Kit

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  • Alternative name

    Human CAM 120/80 ELISA Kit;Human epithelial cadherin ELISA Kit;Human E-cadherin ELISA Kit;Human uvomorulin ELISA Kit;Human CDHE ELISA Kit;Human UVO ELISA Kit;Human Arc-1 ELISA Kit;Human CD324 ELISA Kit;Human ECAD ELISA Kit;Human LCAM ELISA Kit;Human cadherin 1 ELISA Kit;Human cadherin-1 ELISA Kit;Human E-cadherin 1 ELISA Kit;Human cadherin 1, E-cadherin (epithelial) ELISA Kit;Human cadherin 1, type 1, E-cadherin (epithelial) ELISA Kit;Human calcium-dependent adhesion protein, epithelial ELISA Kit;Human cell-CAM 120/80 ELISA Kit;

  • Catalog
    E005829
  • species
    Human
  • GeneCDH1
  • Other Species Mouse Cdh1 ELISA KitBovine CDH1 ELISA KitCanine CDH1 ELISA KitChicken CDH1 ELISA KitRat Cdh1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human CDH1. No significant cross-reactivity or interference between Human CDH1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.56 ng/ml
  • Intended UseHuman CDH1 ELISA Kit allows for the in vitro quantitative determination of CDH1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Introduction: Cadherins (Calcium dependent adhesion molecules) are a class of type-1 transmembrane proteins. They play important roles in cell adhesion, ensuring that cells within tissues are bound together. They are dependent on calcium (Ca2+) ions to function, hence their name. Different members of the cadherin family are found in different locations. E-cadherins are found in epithelial tissue; N-cadherins are found in neurons; and P-cadherins are found in the placenta. T-cadherins have no cytoplasmic domains and must be tethered to the plasma membrane. E-cadherin (epithelial) is the most well-studied member of the family. It consists of 5 cadherin repeats (EC1 ~ EC5) in the extracellular domain, one transmembrane domain, and an intracellular domain that binds p120-catenin and beta-catenin. The intracellular domain contains a highly-phosphorylated region vital to beta-catenin binding and therefore to E-cadherin function. Beta-catenin can also bind to alpha-catenin. Alpha-catenin participates in regulation of actin-containing cytoskeletal filaments. In epithelial cells, E-cadherin-containing cell-to-cell junctions are often adjacent to actin-containing filaments of the cytoskeleton. E-cadherin is first expressed in the 2-cell stage of mammalian development, and becomes phosphorylated by the 8-cell stage, where it causes compaction. In adult tissues, E-cadherin is expressed in epithelial tissues, where it is constantly regenerated with a 5-hour half-life on the cell surface. Loss of E-cadherin function or expression has been implicated in cancer progression and metastasis. E-cadherin downregulation decreases the strength of cellular adhesion within a tissue, resulting in an increase in cellular motility. This in turn may allow cancer cells to cross the basement membrane and invade surrounding tissues. E-cadherin is also used by pathologists to diagnose different kinds of breast cancer. When compared with invasive duct carcinoma, E-cadherin expression is markedly reduced or absent in the great majority of invasive lobular carcinomas when studied by immunohistochemistry. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to E-Cad. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for E-Cad and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain E-Cad, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of E-Cad in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human E-Cad/CTF3 Protein information
  • Uniprot ID CADH1_HUMAN
  • Uniprot AC P12830; A8K1U7; Q13799; Q14216; Q15855; Q16194; Q4PJ14; Q9UII8;
  • UniGene Hs.461086;
  • GeneID 999
  • KEGG hsa:999;
  • Human E-Cad/CTF3 Protein SEQUENCE
  • SEQUENCE 882 AA; 97456 MW; E427118043A13C67 CRC64;

    MGPWSRSLSA LLLLLQVSSW LCQEPEPCHP GFDAESYTFT VPRRHLERGR

    VLGRVNFEDC TGRQRTAYFS LDTRFKVGTD GVITVKRPLR FHNPQIHFLV

    YAWDSTYRKF STKVTLNTVG HHHRPPPHQA SVSGIQAELL TFPNSSPGLR

    RQKRDWVIPP ISCPENEKGP FPKNLVQIKS NKDKEGKVFY SITGQGADTP

    PVGVFIIERE TGWLKVTEPL DRERIATYTL FSHAVSSNGN AVEDPMEILI

    TVTDQNDNKP EFTQEVFKGS VMEGALPGTS VMEVTATDAD DDVNTYNAAI

    AYTILSQDPE LPDKNMFTIN RNTGVISVVT TGLDRESFPT YTLVVQAADL

    QGEGLSTTAT AVITVTDTND NPPIFNPTTY KGQVPENEAN VVITTLKVTD

    ADAPNTPAWE AVYTILNDDG GQFVVTTNPV NNDGILKTAK GLDFEAKQQY

    ILHVAVTNVV PFEVSLTTST ATVTVDVLDV NEAPIFVPPE KRVEVSEDFG

    VGQEITSYTA QEPDTFMEQK ITYRIWRDTA NWLEINPDTG AISTRAELDR

    EDFEHVKNST YTALIIATDN GSPVATGTGT LLLILSDVND NAPIPEPRTI

    FFCERNPKPQ VINIIDADLP PNTSPFTAEL THGASANWTI QYNDPTQESI

    ILKPKMALEV GDYKINLKLM DNQNKDQVTT LEVSVCDCEG AAGVCRKAQP

    VEAGLQIPAI LGILGGILAL LILILLLLLF LRRRAVVKEP LLPPEDDTRD

    NVYYYDEEGG GEEDQDFDLS QLHRGLDARP EVTRNDVAPT LMSVPRYLPR

    PANPDEIGNF IDENLKAADT DPTAPPYDSL LVFDYEGSGS EAASLSSLNS

    SESDKDQDYD YLNEWGNRFK KLADMYGGGE DD

  • UCSC uc002ewg.2; human. [P12830-1];


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