Contact Us
  • Alternative name

    Pituitary adenylate cyclase-activating polypeptide ELISA KIT; PRP-48ADCYAP1 ELISA KIT; PACAP ELISA KIT; PACAP-27 ELISA KIT; PACAP27 ELISA KIT; PACAP-38 ELISA KIT; PACAP38 ELISA KIT

  • Catalog
  • species
  • GenePACAP
  • Standard CurveHuman PACAP ELISA Kit
  • Other Species Human PACAP-27 ELISA KitHuman PACAP-38 ELISA KitMouse PACAP-27 ELISA KitMouse PACAP ELISA KitMouse PACAP-38 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman PACAP ELISA Kit allows for the in vitro quantitative determination of PACAP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilySignal Transduction
  • Product Description
    Principle of the Assay: PACAP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PACAP antibody and an PACAP-HRP conjugate. The assay sample and buffer are incubated together with PACAP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PACAP concentration since PACAP from samples and PACAP-HRP conjugate compete for the anti-PACAP antibody binding site. Since the number of sites is limited, as more sites are occupied by PACAP from the sample, fewer sites are left to bind PACAP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PACAP concentration in each sample is interpolated from this standard curve.

Inquiry About Human PACAP ELISA Kit If you hope to order it or contact us directly, please contact us via We will get back to you in 12hrs,Thanks!