Mouse IL-12p35 ELISA Kit

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  • Alternative name

    Interleukin 12 polypeptide 35 ELISA KIT; Interleukin 12 polypeptide 35IL12 ELISA KIT;

  • Catalog
    E057173
  • species
    Mouse
  • GeneIL-12p35
  • Standard CurveMouse IL-12p35 ELISA Kit
  • Other Species Human IL 12p35 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseMouse IL-12p35 ELISA Kit allows for the in vitro quantitative determination of IL-12p35 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyImmunology
  • Product Description
    specifical
    For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate INTENDED USE This IL-12P35 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse IL-12P35. This ELISA kit for research use only, not for therapeutic or diagnostic applications! PRINCIPLE OF THE ASSAY IL-12P35 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IL-12P35 antibody and an IL-12P35-HRP conjugate. The assay sample and buffer are incubated together with IL-12P35-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-12P35 concentration since IL-12P35 from samples and IL-12P35-HRP conjugate compete for the anti-IL-12P35 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-12P35 from the sample, fewer sites are left to bind IL-12P35-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-12P35 concentration in each sample is interpolated from this standard curve.



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