Mouse AZU/HBP ELISA Kit

Contact Us
  • Alternative name

    Fibroblast growth factor-binding protein 1 ELISA KIT; 17 kDa heparin-binding growth factor-binding protein ELISA KIT; 17 kDa HBGF-binding protein ELISA KIT; HBp17FGFBP1 ELISA KIT; FGFBP ELISA KIT; HBP17 ELISA KIT; FGF-BP ELISA KIT; FGF-BP1 ELISA KIT; FGF-binding protein 1 ELISA KIT; FGFBP-1 ELISA KIT; 17 kDa HBGF-binding protein ELISA KIT; HBp17 ELISA KIT

  • Catalog
    E055507
  • species
    Mouse
  • GeneAZU/HBP
  • Standard CurveMouse AZU/HBP ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of HBP. No significant cross-reactivity or interference between HBP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HBP and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse AZU/HBP ELISA Kit allows for the in vitro quantitative determination of AZU/HBP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the assay: HBP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HBP antibody and an HBP-HRP conjugate. The assay sample and buffer are incubated together with HBP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HBP concentration since HBP from samples and HBP-HRP conjugate compete for the anti-HBP antibody binding site. Since the number of sites is limited, as more sites are occupied by HBP from the sample, fewer sites are left to bind HBP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HBP concentration in each sample is interpolated from this standard curve.



Inquiry About Mouse AZU/HBP ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!