Intended UseMouse ILGF-beta1 ELISA Kit allows for the in vitro quantitative determination of ILGF-beta1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Categories/FamilySignal Transduction
Product Description specificalFor Samples: Cell culture fluid, body fluid, tissue homogenate, serum and blood plasma
Intended Uses: This PRO-IL-1BETA ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of PRO-IL-1BETA in the sample, this PRO-IL-1BETA ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus PRO-IL-1BETA concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of PRO-IL-1BETA utilizes a multiclonal anti-PRO-IL-1BETA antibody and an PRO-IL-1BETA-HRP conjugate. The assay sample and buffer are incubated together with PRO-IL-1BETA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PRO-IL-1BETA concentration since PRO-IL-1BETA from samples and PRO-IL-1BETA-HRP conjugate compete for the anti-PRO-IL-1BETA antibody binding site. Since the number of sites is limited, as more sites are occupied by PRO-IL-1BETA from the sample, fewer sites are left to bind PRO-IL-1BETA-HRP conjugate. Standards of known PRO-IL-1BETA concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of PRO-IL-1BETA. The PRO-IL-1BETA concentration in each sample is interpolated from this standard curve.