Human ALAD ELISA Kit

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  • Alternative name

    Human ALADH ELISA Kit;Human porphobilinogen synthase ELISA Kit;Human PBGS ELISA Kit;Human aminolevulinate dehydratase ELISA Kit;Human delta-aminolevulinic acid dehydratase ELISA Kit;Human aminolevulinate, delta-, dehydratase ELISA Kit;Human testicular tissue protein Li 95 ELISA Kit;

  • Catalog
    E001826
  • species
    Human
  • GeneALAD
  • Standard CurveHuman ALAD ELISA Kit
  • Other Species Mouse Alad ELISA KitBovine ALAD ELISA KitMonkey ALAD ELISA KitRat Alad ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ALAD. No significant cross-reactivity or interference between ALAD and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human ALAD is typically less than 0.128ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by addi
  • Intended UseHuman ALAD ELISA Kit allows for the in vitro quantitative determination of ALAD , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ALAD. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ALAD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ALAD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ALAD in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Delta-aminolevulinic acid dehydratase Protein information
  • Uniprot ID HEM2_HUMAN
  • Uniprot AC P13716; A8K375; B2R6F2; Q16870; Q16871; Q9BVQ9;
  • UniGene Hs.1227;
  • GeneID 210
  • KEGG hsa:210;
  • Human Delta-aminolevulinic acid dehydratase Protein SEQUENCE
  • SEQUENCE 330 AA; 36295 MW; E005F3055F6D9403 CRC64;

    MQPQSVLHSG YFHPLLRAWQ TATTTLNASN LIYPIFVTDV PDDIQPITSL

    PGVARYGVKR LEEMLRPLVE EGLRCVLIFG VPSRVPKDER GSAADSEESP

    AIEAIHLLRK TFPNLLVACD VCLCPYTSHG HCGLLSENGA FRAEESRQRL

    AEVALAYAKA GCQVVAPSDM MDGRVEAIKE ALMAHGLGNR VSVMSYSAKF

    ASCFYGPFRD AAKSSPAFGD RRCYQLPPGA RGLALRAVDR DVREGADMLM

    VKPGMPYLDI VREVKDKHPD LPLAVYHVSG EFAMLWHGAQ AGAFDLKAAV

    LEAMTAFRRA GADIIITYYT PQLLQWLKEE

  • UCSC uc011lxf.3; human. [P13716-1];




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