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Alternative name
Inhibin beta A chain ELISA KIT; Activin beta-A chain ELISA KIT; Erythroid differentiation proteinINHBA ELISA KIT; EDF ELISA KIT
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Catalog
E056610
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species
Mouse
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GeneINB-betaA
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SpecificitySensitivity: The sensitivity in this assay is 1.0 pg/mL.
Specificity: This assay has high sensitivity and excellent specificity for detection of INB-BA. No significant cross-reactivity or interference between INB-BA and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Intended UseMouse INB-betaA ELISA Kit allows for the in vitro quantitative determination of INB-betaA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyMouse ELISA Kit
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Product Description
specificalFor Samples: Cell culture fluid, body fluid, tissue homogenate, serum and blood plasma
Intended Uses: This INB-BA ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of INB-BA in the sample, this INB-BA ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus INB-BA concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of INB-BA utilizes a multiclonal anti-INB-BA antibody and an INB-BA-HRP conjugate. The assay sample and buffer are incubated together with INB-BA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the INB-BA concentration since INB-BA from samples and INB-BA-HRP conjugate compete for the anti-INB-BA antibody binding site. Since he number of sites is limited, as more sites are occupied by INB-BA from the sample, fewer sites are left to bind INB-BA-HRP conjugate. Standards of known INB-BA concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of INB-BA. The INB-BA concentration in each sample is interpolated from this standard curve.
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