Human CHRM5 ELISA Kit

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  • Alternative name

    Human HM5 ELISA Kit;Human cholinergic receptor muscarinic 5 ELISA Kit;Human muscarinic acetylcholine receptor M5 ELISA Kit;Human acetylcholine receptor, muscarinic 5 ELISA Kit;

  • Catalog
    E008272
  • species
    Human
  • GeneCHRM5
  • Other Species Mouse Chrm5 ELISA KitRat Chrm5 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human CHRM5. No significant cross-reactivity or interference between human CHRM5 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human CHRM5 is typically less than 9.75 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
  • Intended UseHuman CHRM5 ELISA Kit allows for the in vitro quantitative determination of CHRM5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CHRM5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CHRM5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHRM5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHRM5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Muscarinic acetylcholine receptor M5 Protein information
  • Uniprot ID ACM5_HUMAN
  • Uniprot AC P08912; Q96RG7;
  • UniGene Hs.584747;
  • GeneID 1133
  • KEGG hsa:1133;
  • Human Muscarinic acetylcholine receptor M5 Protein SEQUENCE
  • SEQUENCE 532 AA; 60074 MW; EE846186F645DD9B CRC64;

    MEGDSYHNAT TVNGTPVNHQ PLERHRLWEV ITIAAVTAVV SLITIVGNVL

    VMISFKVNSQ LKTVNNYYLL SLACADLIIG IFSMNLYTTY ILMGRWALGS

    LACDLWLALD YVASNASVMN LLVISFDRYF SITRPLTYRA KRTPKRAGIM

    IGLAWLISFI LWAPAILCWQ YLVGKRTVPL DECQIQFLSE PTITFGTAIA

    AFYIPVSVMT ILYCRIYRET EKRTKDLADL QGSDSVTKAE KRKPAHRALF

    RSCLRCPRPT LAQRERNQAS WSSSRRSTST TGKPSQATGP SANWAKAEQL

    TTCSSYPSSE DEDKPATDPV LQVVYKSQGK ESPGEEFSAE ETEETFVKAE

    TEKSDYDTPN YLLSPAAAHR PKSQKCVAYK FRLVVKADGN QETNNGCHKV

    KIMPCPFPVA KEPSTKGLNP NPSHQMTKRK RVVLVKERKA AQTLSAILLA

    FIITWTPYNI MVLVSTFCDK CVPVTLWHLG YWLCYVNSTV NPICYALCNR

    TFRKTFKMLL LCRWKKKKVE EKLYWQGNSK LP

  • UCSC uc001zhk.2; human.;




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