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Human ARC ELISA Kit

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  • Alternative name

    Human ARC/ARG3.1 ELISA Kit;Human activity-regulated gene 3.1 protein homolog ELISA Kit;Human Arg3.1 ELISA Kit;Human activity regulated cytoskeleton associated protein ELISA Kit;Human activity-regulated cytoskeleton-associated protein ELISA Kit;
  • Catalog
    E000550
  • species
    Human
  • GeneARC
  • Standard CurveHuman ARC ELISA Kit
  • Other Species Mouse Arc ELISA KitChicken ARC ELISA KitRat Arc ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ARC. No significant cross-reactivity or interference between ARC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ARC and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman ARC ELISA Kit allows for the in vitro quantitative determination of ARC , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical    Intended Uses: This ARC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ARC. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: ARC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ARC antibody and an ARC-HRP conjugate. The assay sample and buffer are incubated together with ARC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ARC concentration since ARC from samples and ARC-HRP conjugate compete for the anti-ARC antibody binding site. Since the number of sites is limited, as more sites are occupied by ARC from the sample, fewer sites are left to bind ARC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ARC concentration in each sample is interpolated from this standard curve.
  • Human Activity-regulated cytoskeleton-associated protein Protein information
  • Uniprot ID ARC_HUMAN
  • Uniprot AC Q7LC44; Q9UJW6; Q9Y469;
  • UniGene Hs.40888; Hs.743662;
  • GeneID 23237
  • KEGG hsa:23237;
  • Human Activity-regulated cytoskeleton-associated protein Protein SEQUENCE

  • SEQUENCE 396 AA; 45316 MW; D5C8A8A07FE9615D CRC64;

    MELDHRTSGG LHAYPGPRGG QVAKPNVILQ IGKCRAEMLE HVRRTHRHLL

    AEVSKQVERE LKGLHRSVGK LESNLDGYVP TSDSQRWKKS IKACLCRCQE

    TIANLERWVK REMHVWREVF YRLERWADRL ESTGGKYPVG SESARHTVSV

    GVGGPESYCH EADGYDYTVS PYAITPPPAA GELPGQEPAE AQQYQPWVPG

    EDGQPSPGVD TQIFEDPREF LSHLEEYLRQ VGGSEEYWLS QIQNHMNGPA

    KKWWEFKQGS VKNWVEFKKE FLQYSEGTLS REAIQRELDL PQKQGEPLDQ

    FLWRKRDLYQ TLYVDADEEE IIQYVVGTLQ PKLKRFLRHP LPKTLEQLIQ

    RGMEVQDDLE QAAEPAGPHL PVEDEAETLT PAPNSESVAS DRTQPE

  • UCSC uc003ywn.2; human.;


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