Mouse Grb14 ELISA Kit

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  • Alternative name

    Mouse GRB14 adapter protein ELISA Kit;Mouse AI505286 ELISA Kit;Mouse growth factor receptor bound protein 14 ELISA Kit;Mouse growth factor receptor-bound protein 14 ELISA Kit;

  • Catalog
    E054828
  • species
    Mouse
  • GeneGrb14
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse Grb14. No significant cross-reactivity or interference between Mouse Grb14 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse Grb14 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseMouse Grb14 ELISA Kit allows for the in vitro quantitative determination of Grb14 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of Grb14 in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse Grb14. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse Grb14 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Grb14, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse Grb14. You can calculate the concentration of Mouse Grb14 in the samples by comparing the OD of the samples to the standard curve.
  • Mouse Growth factor receptor-bound protein 14 Protein information
  • Uniprot ID GRB14_MOUSE
  • Uniprot AC Q9JLM9; Q3UI77; Q8VDI2; Q9CR03;
  • UniGene Mm.214554;
  • GeneID 50915
  • KEGG mmu:50915;
  • Mouse Growth factor receptor-bound protein 14 Protein SEQUENCE
  • SEQUENCE 538 AA; 60573 MW; 04ABD6CEB6ABC6CB CRC64;

    MTTSLQDGQS AAGRAGAQDS PLAVQVCRVA QGKGDAQDPA QVPGLHALSP

    ASDATLRGAI DRRKMKDLDV LEKPPIPNPF PELCCSPLTS VLSAGLFPRA

    NSRKKQVIKV YSEDETSRAL EVPSDITARD VCQLLILKNH YVDDNSWTLF

    EHLSHIGLER TVEDHELPTE VLSHWGVEED NKLYLRKNYA KYEFFKNPMY

    FFPEHMVSFA AEMNGDRSPT QILQVFLSSS TYPEIHGFLH AKEQGKKSWK

    KAYFFLRRSG LYFSTKGTSK EPRHLQLFSE FSTSHVYMSL AGKKKHGAPT

    PYGFCLKPNK AGGPRDLKML CAEEEQSRTC WVTAIRLLKD GMQLYQNYMH

    PYQGRSACNS QSMSPMRSVS ENSLVAMDFS GEKSRVIDNP TEALSVAVEE

    GLAWRKKGCL RLGNHGSPSA PSQSSAVNMA LHRSQPWFHH RISRDEAQRL

    IIRQGPVDGV FLVRDSQSNP RTFVLSMSHG QKIKHYQIIP VEDDGELFHT

    LDDGHTKFTD LIQLVEFYQL NRGVLPCKLK HYCARMAV

  • UCSC uc008jvx.2; mouse.;



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