Intended UseMouse Glycam1 ELISA Kit allows for the in vitro quantitative determination of Glycam1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GlyCAM-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GlyCAM-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GlyCAM-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of GlyCAM-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse Glycosylation-dependent cell adhesion molecule 1 Protein information
Uniprot ID
GLCM1_MOUSE
Uniprot AC
Q02596; A6H6D0;
UniGene
Mm.219621;
GeneID
14663
KEGG
mmu:14663;
Mouse Glycosylation-dependent cell adhesion molecule 1 Protein SEQUENCE