Mouse Gad1 ELISA Kit

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  • Alternative name

    Mouse 67 kDa glutamic acid decarboxylase ELISA Kit;Mouse GAD-67 ELISA Kit;Mouse glutamate decarboxylase 67 kDa isoform ELISA Kit;Mouse Gad67 ELISA Kit;Mouse EP10 ELISA Kit;Mouse Gad-1 ELISA Kit;Mouse glutamate decarboxylase 1 ELISA Kit;Mouse glutamic acid decarboxylase 1 ELISA Kit;

  • Catalog
    E054333
  • species
    Mouse
  • GeneGad1
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.234ng/mL
  • Intended UseMouse Gad1 ELISA Kit allows for the in vitro quantitative determination of Gad1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse Gad1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Gad1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Gad1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Gad1 can be calculated.
  • Mouse Glutamate decarboxylase 1 Protein information
  • Uniprot ID DCE1_MOUSE
  • Uniprot AC P48318; O08685;
  • UniGene Mm.272120;
  • GeneID 14415
  • KEGG mmu:14415;
  • Mouse Glutamate decarboxylase 1 Protein SEQUENCE
  • SEQUENCE 593 AA; 66648 MW; 82D1AAF216F25100 CRC64;

    MASSTPSPAT SSNAGADPNT TNLRPTTYDT WCGVAHGCTR KLGLKICGFL

    QRTNSLEEKS RLVSAFRERQ SSKNLLSCEN SDQGARFRRT ETDFSNLFAQ

    DLLPAKNGEE QTAQFLLEVV DILLNYVRKT FDRSTKVLDF HHPHQLLEGM

    EGFNLELSDH PESLEQILVD CRDTLKYGVR TGHPRFFNQL STGLDIIGLA

    GEWLTSTANT NMFTYEIAPV FVLMEQITLK KMREIVGWSN KDGDGIFSPG

    GAISNMYSIM AARYKYFPEV KTKGMAAVPK LVLFTSEHSH YSIKKAGAAL

    GFGTDNVILI KCNERGKIIP ADLEAKILDA KQKGYVPLYV NATAGTTVYG

    AFDPIQEIAD ICEKYNLWLH VDAAWGGGLL MSRKHRHKLS GIERANSVTW

    NPHKMMGVLL QCSAILVKEK GILQGCNQMC AGYLFQPDKQ YDVSYDTGDK

    AIQCGRHVDI FKFWLMWKAK GTVGFENQIN KCLELADYLY AKIKNREEFE

    MVFDGEPEHT NVCFWYIPQS LRGVPDSPER REKLHRVAPK IKALMMESGT

    TMVGYQPQGD KANFFRMVIS NPAATQSDID FLIEEIERLG QDL

  • UCSC uc008jzk.1; mouse.;



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