Human CCK ELISA Kit

Alternative name
Human CCK ELISA Kit;Human CCK58 ELISA Kit;Human (1-49)-CCK58 ELISA Kit;Human CCK39 ELISA Kit;Human CCK33 ELISA Kit;Human CCK25 ELISA Kit;Human CCK18 ELISA Kit;Human CCK12 ELISA Kit;Human CCK8 ELISA Kit;Human CCK7 ELISA Kit;Human CCK5 ELISA Kit;Human cholecystokinin ELISA Kit;Human cholecystokinin triacontatriapeptide ELISA Kit;Human prepro-cholecystokinin ELISA Kit;
Catalog
E008191
species
Human
GeneCCK
SpecificityThis assay has high sensitivity and excellent specificity for detection of CCK. No significant cross-reactivity or interference between CCK and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CCK and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0pg/mL.
Intended UseHuman CCK ELISA Kit allows for the in vitro quantitative determination of CCK , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalIntended Uses: This CCK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CCK. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||CCK ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CCK. Standards or samples are then added to the microtiter plate wells and CCK if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CCK present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CCK are added to each well to "sandwich" the CCK immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CCK and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCK concentration in each sample is interpolated from this standard curve.
Human Cholecystokinin-5 Protein information
Uniprot ID CCKN_HUMAN
Uniprot AC P06307;
UniGene Hs.458426;
GeneID 885
KEGG hsa:885;
Human Cholecystokinin-5 Protein SEQUENCE
SEQUENCE 115 AA; 12669 MW; 8A3EE6442FAEAF4B CRC64;
MNSGVCLCVL MAVLAAGALT QPVPPADPAG SGLQRAEEAP RRQLRVSQRT
DGESRAHLGA LLARYIQQAR KAPSGRMSIV KNLQNLDPSH RISDRDYMGW
MDFGRRSAEE YEYPS

Human CCK ELISA Kit

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