Mouse Flg ELISA Kit

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  • Catalog
    E053166
  • species
    Mouse
  • GeneFlg
  • Other Species Human FLG ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse FLG. No significant cross-reactivity or interference between mouse FLG and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of mouse FLG is typically less than 0.78 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
  • Intended UseMouse Flg ELISA Kit allows for the in vitro quantitative determination of Flg , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FLG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FLG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FLG is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FLG bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Mouse Filaggrin Protein information
  • Uniprot ID FILA_MOUSE
  • Uniprot AC P11088;
  • UniGene Mm.162684; Mm.439710;
  • Mouse Filaggrin Protein SEQUENCE
  • SEQUENCE 336 AA; 35678 MW; 259F124D3AC0DB2D CRC64;

    PDGSGRSSNR RDRPRQLSPS QSSDSQVHSG VQVEGRRGHS SSANRRAGSS

    SGSGVQGASA GGLAADASRR SGARQGQASA QGRAGSQGQA QGRVSSSADR

    QGRRGVSESR ASDSEGHSDF SEGQAVGAHR QSGAGQRHEQ RSSRGQHGSG

    YYYEQEHSEE ESDSQHQHGH QHEQQRGHQH QHQHQHEHEQ PESGHRQQQS

    SGRGHQGAHQ EQGRDSARPR GSNQGHSSSR HQADSPRVSA RSGSGGRGQS

    PDASGRSSNR RDRPRQPSPS QSSDSQVHSG VQVEAQRGQS SSANRRAGSS

    SGSGVQGAAA SGQGGYESIF TAKHLDFNQS HSYYYY



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