Mouse Frs2 ELISA Kit

Alternative name
Mouse FGFR substrate 2 ELISA Kit;Mouse FGFR-signaling adaptor SNT ELISA Kit;Mouse FRS2-alpha ELISA Kit;Mouse suc1-associated neurotrophic factor target 1 ELISA Kit;Mouse SNT-1 ELISA Kit;Mouse Frs2a ELISA Kit;Mouse 4732458E18 ELISA Kit;Mouse C330018A15Rik ELISA Kit;Mouse Frs2alpha ELISA Kit;Mouse SNT1 ELISA Kit;Mouse fibroblast growth factor receptor substrate 2 ELISA Kit;
Catalog
E053075
species
Mouse
GeneFrs2
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseMouse Frs2 ELISA Kit allows for the in vitro quantitative determination of Frs2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalFor samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate 1 INTENDED USE This FRS2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse FRS2. This ELISA kit for research use only, not for therapeutic or diagnostic applications! PRINCIPLE OF THE ASSAY FRS2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FRS2 antibody and an FRS2-HRP conjugate. The assay sample and buffer are incubated together with FRS2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FRS2 concentration since FRS2 from samples and FRS2-HRP conjugate compete for the anti-FRS2 antibody binding site. Since the number of sites is limited, as more sites are occupied by FRS2 from the sample, fewer sites are left to bind FRS2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FRS2 concentration in each sample is interpolated from this standard curve.
Mouse Fibroblast growth factor receptor substrate 2 Protein information
Uniprot ID FRS2_MOUSE
Uniprot AC Q8C180;
UniGene Mm.135965;
GeneID 327826
KEGG mmu:327826;
Mouse Fibroblast growth factor receptor substrate 2 Protein SEQUENCE
SEQUENCE 508 AA; 56794 MW; DFFE8A818BFF8631 CRC64;
MGSCCSCPDK DTVPDNHRNK FKVINVDDDG NELGSGVMEL TDTELILYTR
KRDSVKWHYL CLRRYGYDSN LFSFESGRRC QTGQGIFAFK CARAEELFNM
LQEIMQNNSI NVVEEPVVER SSHQTELEVP RTPRTPTTPG LGAQNLPNGY
PRYPSFGDAS SHPSSRHPSV GSARLPSVGE ESTHPLLVAE EQVHTYVNTT
GVQEERKNRA SVHVPPEARV SNAESNTPKE EPSNPEDRDP QVLLKPEGVR
FVLGPTPVQK QLMEKEKLEQ LGKDPVSGSG AGNTEWDTGY DSDERRDVPP
VNKLVYENIN GLSIPSASGV RRGRLTSTST SDTQNINNSA QRRPALLNYE
NLPSLPPVWE ARKLSRDEDD NLGPKTPSLN GYHNNLDPMH NYVNTENVTV
PASAHKIDYS KRRDCTPTVF NFDIRRPSLE HRQLNYIQVD LEGGSDSDNP
QTPKTPTTPL PQTPTRRTEL YAVIDIERTA AMSNLQKALP RDDGTSRKTR
HNSTDLPM
UCSC uc007hct.1; mouse.;

Category

Mouse Frs2 ELISA Kit