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Human MAP2K1 ELISA Kit

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  • Alternative name

    Human MAP kinase kinase 1 ELISA Kit;Human MAPKK 1 ELISA Kit;Human MKK1 ELISA Kit;Human ERK activator kinase 1 ELISA Kit;Human MAPK/ERK kinase 1 ELISA Kit;Human MEK 1 ELISA Kit;Human MEK1 ELISA Kit;Human PRKMK1 ELISA Kit;Human CFC3 ELISA Kit;Human MAPKK1 ELISA Kit;Human mitogen-activated protein kinase kinase 1 ELISA Kit;Human dual specificity mitogen-activated protein kinase kinase 1 ELISA Kit;Human protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1) ELISA Kit;
  • Catalog
    E024708
  • species
    Human
  • GeneMAP2K1
  • Standard CurveHuman MAP2K1 ELISA Kit
  • Other Species Mouse Map2k1 ELISA KitHamster MAP2K1 ELISA KitRabbit MAP2K1 ELISA KitRat Map2k1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mitogen Activated Protein Kinase Kinase 1 (MAP2K1). No significant cross-reactivity or interference between Mitogen Activated Protein Kinase Kinase 1 (MAP2K1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.056ng/mL.
  • Intended UseHuman MAP2K1 ELISA Kit allows for the in vitro quantitative determination of MAP2K1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mitogen Activated Protein Kinase Kinase 1 (MAP2K1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mitogen Activated Protein Kinase Kinase 1 (MAP2K1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mitogen Activated Protein Kinase Kinase 1 (MAP2K1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mitogen Activated Protein Kinase Kinase 1 (MAP2K1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Dual specificity mitogen-activated protein kinase kinase 1 Protein information
  • Uniprot ID MP2K1_HUMAN
  • Uniprot AC Q02750;
  • UniGene Hs.145442; Hs.677223;
  • GeneID 5604
  • KEGG hsa:5604;
  • Human Dual specificity mitogen-activated protein kinase kinase 1 Protein SEQUENCE

  • SEQUENCE 393 AA; 43439 MW; 0344118FFC842D51 CRC64;

    MPKKKPTPIQ LNPAPDGSAV NGTSSAETNL EALQKKLEEL ELDEQQRKRL

    EAFLTQKQKV GELKDDDFEK ISELGAGNGG VVFKVSHKPS GLVMARKLIH

    LEIKPAIRNQ IIRELQVLHE CNSPYIVGFY GAFYSDGEIS ICMEHMDGGS

    LDQVLKKAGR IPEQILGKVS IAVIKGLTYL REKHKIMHRD VKPSNILVNS

    RGEIKLCDFG VSGQLIDSMA NSFVGTRSYM SPERLQGTHY SVQSDIWSMG

    LSLVEMAVGR YPIPPPDAKE LELMFGCQVE GDAAETPPRP RTPGRPLSSY

    GMDSRPPMAI FELLDYIVNE PPPKLPSGVF SLEFQDFVNK CLIKNPAERA

    DLKQLMVHAF IKRSDAEEVD FAGWLCSTIG LNQPSTPTHA AGV

  • UCSC uc010bhq.4; human. [Q02750-1];


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