SpecificityThis assay has high sensitivity and excellent specificity for detection of DEF5. No significant cross-reactivity or interference between DEF5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DEF5 and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseMouse DEF5 ELISA Kit allows for the in vitro quantitative determination of DEF5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalIntended Uses: This DEF5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse DEF5. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay||DEF5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DEF5 antibody and an DEF5-HRP conjugate. The assay sample and buffer are incubated together with DEF5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DEF5 concentration since DEF5 from samples and DEF5-HRP conjugate compete for the anti-DEF5 antibody binding site. Since the number of sites is limited, as more sites are occupied by DEF5 from the sample, fewer sites are left to bind DEF5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DEF5 concentration in each sample is interpolated from this standard curve.