Human FZD1 ELISA Kit

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  • Alternative name

    Human fz-1 ELISA Kit;Human hFz1 ELISA Kit;Human fzE1 ELISA Kit;Human frizzled class receptor 1 ELISA Kit;Human frizzled-1 ELISA Kit;Human Wnt receptor ELISA Kit;Human frizzled 1, seven transmembrane spanning receptor ELISA Kit;Human frizzled family receptor 1 ELISA Kit;Human frizzled homolog 1 ELISA Kit;Human frizzled, Drosophila, homolog of, 1 ELISA Kit;

  • Catalog
    E015567
  • species
    Human
  • GeneFZD1
  • Standard CurveHuman FZD1 ELISA Kit
  • Other Species Mouse Fzd1 ELISA KitChicken FZD1 ELISA KitRat Fzd1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Frizzled Homolog 1 (FZD1). No significant cross-reactivity or interference between Frizzled Homolog 1 (FZD1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.053ng/mL.
  • Intended UseHuman FZD1 ELISA Kit allows for the in vitro quantitative determination of FZD1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Frizzled Homolog 1 (FZD1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Frizzled Homolog 1 (FZD1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Frizzled Homolog 1 (FZD1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Frizzled Homolog 1 (FZD1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Frizzled-1 Protein information
  • Uniprot ID FZD1_HUMAN
  • Uniprot AC Q9UP38; A4D1E8; O94815; Q549T8;
  • UniGene Hs.94234;
  • GeneID 8321
  • KEGG hsa:8321;
  • Human Frizzled-1 Protein SEQUENCE
  • SEQUENCE 647 AA; 71158 MW; 7FC916A736482826 CRC64;

    MAEEEAPKKS RAAGGGASWE LCAGALSARL AEEGSGDAGG RRRPPVDPRR

    LARQLLLLLW LLEAPLLLGV RAQAAGQGPG QGPGPGQQPP PPPQQQQSGQ

    QYNGERGISV PDHGYCQPIS IPLCTDIAYN QTIMPNLLGH TNQEDAGLEV

    HQFYPLVKVQ CSAELKFFLC SMYAPVCTVL EQALPPCRSL CERARQGCEA

    LMNKFGFQWP DTLKCEKFPV HGAGELCVGQ NTSDKGTPTP SLLPEFWTSN

    PQHGGGGHRG GFPGGAGASE RGKFSCPRAL KVPSYLNYHF LGEKDCGAPC

    EPTKVYGLMY FGPEELRFSR TWIGIWSVLC CASTLFTVLT YLVDMRRFSY

    PERPIIFLSG CYTAVAVAYI AGFLLEDRVV CNDKFAEDGA RTVAQGTKKE

    GCTILFMMLY FFSMASSIWW VILSLTWFLA AGMKWGHEAI EANSQYFHLA

    AWAVPAIKTI TILALGQVDG DVLSGVCFVG LNNVDALRGF VLAPLFVYLF

    IGTSFLLAGF VSLFRIRTIM KHDGTKTEKL EKLMVRIGVF SVLYTVPATI

    VIACYFYEQA FRDQWERSWV AQSCKSYAIP CPHLQAGGGA PPHPPMSPDF

    TVFMIKYLMT LIVGITSGFW IWSGKTLNSW RKFYTRLTNS KQGETTV

  • UCSC uc003ula.4; human.;



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