SpecificityThis assay has high sensitivity and excellent specificity for detection of CYP2A6. No significant cross-reactivity or interference between CYP2A6 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CYP2A6 and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseMouse CYP2A6 ELISA Kit allows for the in vitro quantitative determination of CYP2A6 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalIntended Uses: This CYP2A6 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CYP2A6. This ELISA kit for research use only,not for therapeutic or diagnostic applications!
Principle of the Assay: CYP2A6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-CYP2A6 antibody and an CYP2A6-HRP conjugate. The assay sample and buffer are incubated together with CYP2A6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CYP2A6 concentration since CYP2A6 from samples and CYP2A6-HRP conjugate compete for the anti-CYP2A6 antibody binding site. Since the number of sites is limited, as more sites are occupied by CYP2A6 from the sample, fewer sites are left to bind CYP2A6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CYP2A6 concentration in each sample is interpolated from this standard curve.