Mouse CYP2A6 ELISA Kit

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  • Alternative name

    Cytochrome P450 2A6 ELISA KIT; 1,4-cineole 2-exo-monooxygenase ELISA KIT; CYPIIA6 ELISA KIT; Coumarin 7-hydroxylase ELISA KIT; Cytochrome P450 IIA3 ELISA KIT; Cytochrome P450(I)CYP2A6 ELISA KIT; CYP2A3 ELISA KIT

  • Catalog
  • species
  • GeneCYP2A6
  • Standard CurveMouse CYP2A6 ELISA Kit
  • Other Species Human CYP2A6 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CYP2A6. No significant cross-reactivity or interference between CYP2A6 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CYP2A6 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL
  • Intended UseMouse CYP2A6 ELISA Kit allows for the in vitro quantitative determination of CYP2A6 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Intended Uses: This CYP2A6 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CYP2A6. This ELISA kit for research use only,not for therapeutic or diagnostic applications! Principle of the Assay: CYP2A6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-CYP2A6 antibody and an CYP2A6-HRP conjugate. The assay sample and buffer are incubated together with CYP2A6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CYP2A6 concentration since CYP2A6 from samples and CYP2A6-HRP conjugate compete for the anti-CYP2A6 antibody binding site. Since the number of sites is limited, as more sites are occupied by CYP2A6 from the sample, fewer sites are left to bind CYP2A6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CYP2A6 concentration in each sample is interpolated from this standard curve.

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