Human BLVRA ELISA Kit

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  • Alternative name

    Human BVR A ELISA Kit;Human biliverdin-IX alpha-reductase ELISA Kit;Human BLVR ELISA Kit;Human BVR ELISA Kit;Human BVRA ELISA Kit;Human biliverdin reductase A ELISA Kit;Human testis tissue sperm-binding protein Li 61n ELISA Kit;

  • Catalog
    E004983
  • species
    Human
  • GeneBLVRA
  • Standard CurveHuman BLVRA ELISA Kit
  • Other Species Mouse Blvra ELISA KitRat Blvra ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Biliverdin Reductase A (BLVRA). No significant cross-reactivity or interference between Biliverdin Reductase A (BLVRA) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.273ng/mL.
  • Intended UseHuman BLVRA ELISA Kit allows for the in vitro quantitative determination of BLVRA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Biliverdin Reductase A (BLVRA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Biliverdin Reductase A (BLVRA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Biliverdin Reductase A (BLVRA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Biliverdin Reductase A (BLVRA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Biliverdin reductase A Protein information
  • Uniprot ID BIEA_HUMAN
  • Uniprot AC P53004; A8K747; O95019; Q86UX0; Q96QL4; Q9BRW8;
  • UniGene Hs.488143;
  • GeneID 644
  • KEGG hsa:644;
  • Human Biliverdin reductase A Protein SEQUENCE
  • SEQUENCE 296 AA; 33428 MW; 2CF2AA7F1CDDB707 CRC64;

    MNAEPERKFG VVVVGVGRAG SVRMRDLRNP HPSSAFLNLI GFVSRRELGS

    IDGVQQISLE DALSSQEVEV AYICSESSSH EDYIRQFLNA GKHVLVEYPM

    TLSLAAAQEL WELAEQKGKV LHEEHVELLM EEFAFLKKEV VGKDLLKGSL

    LFTAGPLEEE RFGFPAFSGI SRLTWLVSLF GELSLVSATL EERKEDQYMK

    MTVCLETEKK SPLSWIEEKG PGLKRNRYLS FHFKSGSLEN VPNVGVNKNI

    FLKDQNIFVQ KLLGQFSEKE LAAEKKRILH CLGLAEEIQK YCCSRK

  • UCSC uc003tir.4; human.;


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