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Alternative name
Arachidonic Acid ELISA Kit
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Catalog
E003543
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species
Human
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GeneAA
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Other Species
Human AT2R1-AA ELISA KitHuman AGTR2-AA ELISA KitHuman AGTR2 AA ELISA KitHuman AA ELISA KitHuman AA NAT ELISA KitHuman AA-NAT ELISA KitHuman LKM-AA ELISA KitHuman PDGF-AA ELISA KitMouse AT1R-AA ELISA KitMouse AGTR2-AA ELISA KitMouse AA ELISA KitMouse AA-NAT ELISA KitMouse H2-Aa ELISA KitMouse LKM-AA ELISA KitMouse PDGF-AA ELISA KitRat RT1 class I histocompatibility antigen, AA alpha chain ELISA KitBovine AA ELISA KitCanine AA ELISA KitChicken AA ELISA KitGeneral AA ELISA KitPorcine AA ELISA KitRabbit AA ELISA KitRat AA ELISA KitCamel AA ELISA KitGoat AA ELISA KitGuinea Pig AA ELISA KitHamster AA ELISA Kit
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SpecificityNo cross-reaction with other factors
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.938ng/ml
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Intended UseHuman AA ELISA Kit allows for the in vitro quantitative determination of AA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole kit at 4℃
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Product Description
specificalHuman AA ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in th is kit has been pre-coated with an antibody specific to AA , During the reaction,AA in the sample or standard competes with a fixed amount of biotin-labeled AA for sites on a pre-coated Monoclonal antibody specific to AA . Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of AA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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