Mouse Crygf ELISA Kit

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  • Alternative name

    Mouse gamma-F-crystallin ELISA Kit;Mouse 3110001K11Rik ELISA Kit;Mouse Cryg-2 ELISA Kit;Mouse DGcry-2 ELISA Kit;Mouse Len-2 ELISA Kit;Mouse crystallin, gamma F ELISA Kit;Mouse gamma-crystallin F ELISA Kit;

  • Catalog
    E049726
  • species
    Mouse
  • GeneCrygf
  • Standard CurveMouse Crygf ELISA Kit
  • Other Species Bovine CRYGF ELISA KitRat Crygf ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Crystallin Gamma F (CRYgF). No significant cross-reactivity or interference between Crystallin Gamma F (CRYgF) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.055ng/mL.
  • Intended UseMouse Crygf ELISA Kit allows for the in vitro quantitative determination of Crygf , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Crystallin Gamma F (CRYgF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Crystallin Gamma F (CRYgF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Crystallin Gamma F (CRYgF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Crystallin Gamma F (CRYgF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse Gamma-crystallin F Protein information
  • Uniprot ID CRGF_MOUSE
  • Uniprot AC Q9CXV3;
  • UniGene Mm.275362;
  • GeneID 12969
  • KEGG mmu:12969;
  • Mouse Gamma-crystallin F Protein SEQUENCE
  • SEQUENCE 174 AA; 21249 MW; 3483661E528C9E99 CRC64;

    MGKITFYEDR SFQGRHYECS TDHSNLQPYF SRCNSVRVDS GCWMLYEQPN

    FAGCQYFLRR GDYPDYQQWM GFSDSVRSCH LIPHSTSHRI RIYEREDYRG

    QMVEITDDCS HLQDRFHFSD FHSFHVMEGY WVLYEMPNYR GRQYLLRPGE

    YRRYHDWGAM NARVGSLRRI MDFY

  • UCSC uc007bhv.1; mouse.;




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