Human GUK1 ELISA Kit

Alternative name
Human GMP kinase ELISA Kit;Human GMK ELISA Kit;Human guanylate kinase 1 ELISA Kit;Human guanylate kinase ELISA Kit;Human ATP:GMP phosphotransferase ELISA Kit;
Catalog
E017798
species
Human
GeneGUK1
Other Species Mouse Guk1 ELISA Kit Bovine GUK1 ELISA Kit Porcine GUK1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human GUK1. No significant cross-reactivity or interference between Human GUK1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human GUK1 and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseHuman GUK1 ELISA Kit allows for the in vitro quantitative determination of GUK1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GUK1 in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GUK1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human GUK1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GUK1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human GUK1. You can calculate the concentration of Human GUK1 in the samples by comparing the OD of the samples to the standard curve.
Human Guanylate kinase Protein information
Uniprot ID KGUA_HUMAN
Uniprot AC Q16774; B1ANH1;
UniGene Hs.376933;
GeneID 2987
KEGG hsa:2987;
Human Guanylate kinase Protein SEQUENCE
SEQUENCE 197 AA; 21726 MW; C49573212A0DA825 CRC64;
MSGPRPVVLS GPSGAGKSTL LKRLLQEHSG IFGFSVSHTT RNPRPGEENG
KDYYFVTREV MQRDIAAGDF IEHAEFSGNL YGTSKVAVQA VQAMNRICVL
DVDLQGVRNI KATDLRPIYI SVQPPSLHVL EQRLRQRNTE TEESLVKRLA
AAQADMESSK EPGLFDVVII NDSLDQAYAE LKEALSEEIK KAQRTGA
UCSC uc001hsi.4; human. [Q16774-1];

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Category

Human GUK1 ELISA Kit

Human GMP kinase ELISA Kit;Human GMK ELISA Kit;Human guanylate kinase 1 ELISA Kit;Human guanylate kinase ELISA Kit;Human ATP:GMP phosphotransferase ELISA Kit;