Human BID ELISA Kit

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  • Alternative name

    Human p22 BID ELISA Kit;Human BID ELISA Kit;Human p15 BID ELISA Kit;Human p13 BID ELISA Kit;Human p11 BID ELISA Kit;Human FP497 ELISA Kit;Human BH3 interacting domain death agonist ELISA Kit;Human BH3-interacting domain death agonist ELISA Kit;Human BID isoform ES(1b) ELISA Kit;Human BID isoform L(2) ELISA Kit;Human BID isoform Si6 ELISA Kit;Human Human BID coding sequence ELISA Kit;Human apoptic death agonist ELISA Kit;Human desmocollin type 4 ELISA Kit;

  • Catalog
    E004923
  • species
    Human
  • GeneBID
  • Other Species Human BID/TBID ELISA KitMouse Bid ELISA KitChicken BID ELISA KitPorcine BID ELISA KitRat Bid ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of BH3. No significant cross-reactivity or interference between BH3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BH3 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman BID ELISA Kit allows for the in vitro quantitative determination of BID , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical
    Intended Uses: This BH3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human BH3. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||BH3 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for BH3. Standards or samples are then added to the microtiter plate wells and BH3 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of BH3 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for BH3 are added to each well to "sandwich" the BH3 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain BH3 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BH3 concentration in each sample is interpolated from this standard curve.
  • Human BH3-interacting domain death agonist p11 Protein information
  • Uniprot ID BID_HUMAN
  • Uniprot AC P55957; Q549M7; Q71T04; Q7Z4M9; Q8IY86;
  • UniGene Hs.517145; Hs.591054;
  • GeneID 637
  • KEGG hsa:637;
  • Human BH3-interacting domain death agonist p11 Protein SEQUENCE
  • SEQUENCE 195 AA; 21995 MW; B17A07334C1AFBEF CRC64;

    MDCEVNNGSS LRDECITNLL VFGFLQSCSD NSFRRELDAL GHELPVLAPQ

    WEGYDELQTD GNRSSHSRLG RIEADSESQE DIIRNIARHL AQVGDSMDRS

    IPPGLVNGLA LQLRNTSRSE EDRNRDLATA LEQLLQAYPR DMEKEKTMLV

    LALLLAKKVA SHTPSLLRDV FHTTVNFINQ NLRTYVRSLA RNGMD

  • UCSC uc002znc.3; human. [P55957-1];




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