Human RB1 ELISA Kit

Alternative name
Human p105-Rb ELISA Kit;Human pRb ELISA Kit;Human RB ELISA Kit;Human pp110 ELISA Kit;Human OSRC ELISA Kit;Human PPP1R130 ELISA Kit;Human RB transcriptional corepressor 1 ELISA Kit;Human retinoblastoma-associated protein ELISA Kit;Human GOS563 exon 17 substitution mutation causes premature stop ELISA Kit;Human exon 17 tumor GOS561 substitution mutation causes premature stop ELISA Kit;Human prepro-retinoblastoma-associated protein ELISA Kit;Human protein phosphatase 1, regulatory subunit 130 ELISA Kit;Human retinoblastoma 1 ELISA Kit;Human retinoblastoma suspectibility protein ELISA Kit;
Catalog
E032938
species
Human
GeneRB1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human RB1. No significant cross-reactivity or interference between Human RB1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human RB1 and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.094ng/mL
Intended UseHuman RB1 ELISA Kit allows for the in vitro quantitative determination of RB1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of RB1 in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human RB1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human RB1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RB1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human RB1. You can calculate the concentration of Human RB1 in the samples by comparing the OD of the samples to the standard curve.
Human Retinoblastoma-associated protein Protein information
Uniprot ID RB_HUMAN
Uniprot AC P06400; A8K5E3; P78499; Q5VW46; Q8IZL4;
UniGene Hs.408528;
GeneID 5925
KEGG hsa:5925;
Human Retinoblastoma-associated protein Protein SEQUENCE
SEQUENCE 928 AA; 106159 MW; C8E746111E19CC32 CRC64;
MPPKTPRKTA ATAAAAAAEP PAPPPPPPPE EDPEQDSGPE DLPLVRLEFE
ETEEPDFTAL CQKLKIPDHV RERAWLTWEK VSSVDGVLGG YIQKKKELWG
ICIFIAAVDL DEMSFTFTEL QKNIEISVHK FFNLLKEIDT STKVDNAMSR
LLKKYDVLFA LFSKLERTCE LIYLTQPSSS ISTEINSALV LKVSWITFLL
AKGEVLQMED DLVISFQLML CVLDYFIKLS PPMLLKEPYK TAVIPINGSP
RTPRRGQNRS ARIAKQLEND TRIIEVLCKE HECNIDEVKN VYFKNFIPFM
NSLGLVTSNG LPEVENLSKR YEEIYLKNKD LDARLFLDHD KTLQTDSIDS
FETQRTPRKS NLDEEVNVIP PHTPVRTVMN TIQQLMMILN SASDQPSENL
ISYFNNCTVN PKESILKRVK DIGYIFKEKF AKAVGQGCVE IGSQRYKLGV
RLYYRVMESM LKSEEERLSI QNFSKLLNDN IFHMSLLACA LEVVMATYSR
STSQNLDSGT DLSFPWILNV LNLKAFDFYK VIESFIKAEG NLTREMIKHL
ERCEHRIMES LAWLSDSPLF DLIKQSKDRE GPTDHLESAC PLNLPLQNNH
TAADMYLSPV RSPKKKGSTT RVNSTANAET QATSAFQTQK PLKSTSLSLF
YKKVYRLAYL RLNTLCERLL SEHPELEHII WTLFQHTLQN EYELMRDRHL
DQIMMCSMYG ICKVKNIDLK FKIIVTAYKD LPHAVQETFK RVLIKEEEYD
SIIVFYNSVF MQRLKTNILQ YASTRPPTLS PIPHIPRSPY KFPSSPLRIP
GGNIYISPLK SPYKISEGLP TPTKMTPRSR ILVSIGESFG TSEKFQKINQ
MVCNSDRVLK RSAEGSNPPK PLKKLRFDIE GSDEADGSKH LPGESKFQQK
LAEMTSTRTR MQKQKMNDSM DTSNKEEK
UCSC uc001vcb.4; human.;

Category

Human RB1 ELISA Kit