Mouse Col11a1 ELISA Kit

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  • Alternative name

    Mouse C530001D20Rik ELISA Kit;Mouse cho ELISA Kit;Mouse collagen, type XI, alpha 1 ELISA Kit;Mouse collagen alpha-1(XI) chain ELISA Kit;Mouse a1(XI) collagen ELISA Kit;Mouse pro-alpha1(XI) collagen ELISA Kit;Mouse procollagen, type XI, alpha 1 ELISA Kit;

  • Catalog
    E049171
  • species
    Mouse
  • GeneCol11a1
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseMouse Col11a1 ELISA Kit allows for the in vitro quantitative determination of Col11a1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical
    Intended Uses: This COL11A1 ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofCOL11A1 in the sample, thisCOL11A1 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusCOL11A1 concentration. The concentration ofCOL11A1 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: This COL11A1 enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific forCOL11A1. Standards or samples are then added to the microtiter plate wells andCOL11A1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount ofCOL11A1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific forCOL11A1 are added to each well to "sandwich" theCOL11A1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that containCOL11A1 and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
  • Mouse Collagen alpha-1(XI) chain Protein information
  • Uniprot ID COBA1_MOUSE
  • Uniprot AC Q61245; Q64047; Q80WR4;
  • UniGene Mm.209715;
  • GeneID 12814
  • KEGG mmu:12814;
  • Mouse Collagen alpha-1(XI) chain Protein SEQUENCE
  • SEQUENCE 1804 AA; 181032 MW; 918C3D4B8C964470 CRC64;

    MEPWSRWKTK RWIWDLTIST LALTFLFQAR EVRGAAPVDI LKALDFHNSP

    VGISKTTGFC TNRKNSKDPD VAYRVTEEAQ ISAPTKQLFP GGIFPQDFSI

    LFTIKPKKGT QAFLLSLYNE HGIQQLGVEV GRSPVFLFED HTGKPTPENY

    PLFSTVNIAD GKWHRVAISV EKKTVTMIVD CKKKITKPLD RSERSIVDTN

    GIMVFGTRIL ETDVFQGDIQ QFLITGDPKA AYDYCDHYSP DCDLTSKAAQ

    AQEPHIDEYA PEDIIEYDYE YGETDYKEAE SVTEMPTFTE ETVAQTEANI

    VDDFQDYNYG TMEPYQTETP RRVSGSNEPN PVEEGFTEEY LTGEDYDVQR

    NTSEDILYGN KGVDGRDSDL LVDGDLGEYD FYEYKEYEER TTTSPNEEFG

    PGVPAETDFT ETSINGHGAY GEKGQKGEPA VVEPGMLVEG PPGPAGPAGL

    MGPPGLQGPS GLPGDPGDRG PPGRPGLPGA DGLPGPPGTM LMLPFRYGGD

    GSKGPTISAQ EAQAQAILQQ ARIALRGPPG PMGLTGRPGP VGGPGSAGAK

    GESGDPGPQG PRGVQGPPGP TGKPGKRGRP GADGGRGMPG ESGSKGDRGF

    DGLPGLPGDK GHRGERGPQG PPGLPGDDGM RGEDGEIGPR GLPGEAGPRG

    LLGPRGTPGP PGQPGIGGID GPQGPKGNMG PQGEPGPPGQ QGNPGPQGLP

    GPQGPIGPPG EKGPQGKPGL AGLPGADGPP GHPGKEGQSG EKGALGPPGP

    QGPIGYPGPR GVKGADGVRG LKGSKGEKGE DGFPGFKGDM GLKGDRGEVG

    QVGPRGEDGP EGPKGRAGPT GDPGPSGQAG EKGKLGVPGL PGYPGRQGPK

    GSTGFPGFPG ANGEKGARGI AGKPGPRGQR GPTGPRGSRG ARGPTGKPGP

    KGTSGGDGPP GPPGERGPQG PQGPVGFPGP KGPPGPAGKD GLPGHPGQRG

    ETGFQGKTGP PGPGGVVGPQ GPTGETGPIG ERGHPGPPGP PGEQGLPGAA

    GKEGAKGDPG PQGISGKDGP AGIRGFPGER GLPGAQGAPG LKGGEGPQGP

    QGPVGSPGER GSAGTAGPIG LPGRPGPQGP PGPAGEKGAP GEKGPQGPAG

    RDGVQGPVGL PGPAGPAGSP GEDGDKGEIG EPGQKGSKGD KGENGPPGPP

    GLQGPVGAPG IAGGDGEPGP RGQQGMFGQK GDEGARGFPG LPGPIGLQGL

    PGPPGEKGEN GDVGPMGPPG PPGPRGPQGP NGADGPQGPP GSIGSVGVVG

    DKGEPGEAGN PGPPGEAGSG GLKGERGEKG EAGPPGAAGP AGIKGPPGDD

    GPKGNPGPVG FPGDPGPPGE PGPAGQDGVG GDKGEDGDPG QPGPPGPSGE

    AGPPGPPGKR GPPGASGSEG RQGEKGAKGE AGAEGPPGKT GPVGPQGPSG

    KPGPEGLRGI PGPVGEQGLP GAAGQDGPPG PLGPPGLPGL KGDPGSKGEK

    GHPGLIGLIG PPGEQGEKGD RGLPGTQGSP GAKGDGGIPG PAGPIGPPGP

    PGLPGPAGPK GNKGSSGPTG QKGDSGMPGP PGPPGPPGEV IQPLPILSPK

    KTRRHTESIQ GDAGDNILDY SDGMEEIFGS LNSLKQDIEH MKFPMGTQTN

    PARTCKDLQL SHPDFPDGEY WIDPNQGCSG DSFKVYCNFT AGGETCIYPD

    KKSEGVRISS WPKEKPGSWY SEFKRGKLLS YLDVEGNSIN MVQMTFLKLL

    TASARQNFTY NCHQSAAWYD VLSGSYDKAL RFLGSNDEEM SYENNPHIKA

    LYDGCASRKG YEKTVIEINT PKIDQVPIID VMINDFGDQN QKFGFEVGPA

    CFLG

  • UCSC uc008rbk.1; mouse. [Q61245-1];



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